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[人巨细胞病毒感染对人胚肺细胞中HOXB1、HOXB5、HOXB6和HOXB9基因表达的影响]

[Influence of human cytomegalovirus infection on the expressions of HOXB1, HOXB5, HOXB6, and HOXB9 genes in human embryo lung cells].

作者信息

Chen L Y, Wu G J, Dai G

机构信息

Department of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.

出版信息

Hunan Yi Ke Da Xue Xue Bao. 2001 Jun 28;26(3):189-91.

PMID:12536675
Abstract

OBJECTIVE

To study the expressions of HOXB1, HOXB5, HOXB6, and HOXB9 genes in human embryo lung (HEL) cells and influence of human cytomegalovirus (HCMV) infection on the expressions of these genes.

METHOD

The expressions of HOXB1, HOXB5, HOXB6, and HOXB9 genes were detected with semi-quantitative RT-PCR method.

RESULTS

  1. HEL cells expressed HOXB5 and HOXB6 genes, but they did not express HOXB1 and HOXB9 genes. 2. After HCMV infection, HEL cell was induced to express HOXB9 gene, as the expression of HOXB6 gene was elevated. The expression of HOXB5 gene had no significant change. HOXB1 was still not expressed. 3. Treated with all-trans retinoic acid (ATRA), the expression of HOXB9 gene in HEL cells infected by HCMV was significantly increased. But during the advanced infect period, the expression of HOXB6 was significantly decreased.

CONCLUSION

HCMV can induce abnormal expressions of HOXB6 and HOXB9 genes, which may play an important role in HCMV-induced abnormal embryogenesis.

摘要

目的

研究HOXB1、HOXB5、HOXB6和HOXB9基因在人胚肺（HEL）细胞中的表达情况以及人巨细胞病毒（HCMV）感染对这些基因表达的影响。

方法

采用半定量逆转录聚合酶链反应（RT-PCR）法检测HOXB1、HOXB5、HOXB6和HOXB9基因的表达。

结果

  1. HEL细胞表达HOXB5和HOXB6基因，但不表达HOXB1和HOXB9基因。2. HCMV感染后，HEL细胞被诱导表达HOXB9基因，同时HOXB6基因的表达升高。HOXB5基因的表达无明显变化。HOXB1仍未表达。3. 用全反式维甲酸（ATRA）处理后，HCMV感染的HEL细胞中HOXB9基因的表达显著增加。但在感染后期，HOXB6的表达显著下降。

结论

HCMV可诱导HOXB6和HOXB9基因的异常表达，这可能在HCMV诱导的异常胚胎发生中起重要作用。

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