Chen X, Kanekura T, Zhang G Y
Department of Dermatalogy, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Hunan Yi Ke Da Xue Xue Bao. 2001 Aug 28;26(4):297-300.
To investigate the expression of matrix metalloproteinases (MMPs) and its correlation with basigin/CD147 expression in metastatic lesions of cutaneous Sequamous cell carcinoma (SCC).
Expressions of MMPs and basigin/CD147 were examined by DAB and ALP immunohistochemical staining in 14 metastatic SSCs, and immunoblot analysis was used to observe the basigin/CD147 expression in metastatic samples.
Normal skin and lymph nodes were basically negative for MMPs, but expressions of MMP-1, MMP-2, MMP-3 and MTl-MMP were increased in both tumor cells and stromal cells in metastatic SCC. Strongly positive or positive staining was observed in 4 or 8 of metastatic SCCs for basigin/CD147 in tumor cells respectively. Significant correlations were found between basigin/CD147 expression in tumor cells and MMP-1, MMP-2, MMP-3, MTl-MMP expression in surrounding stromal cells (p-values: 0.012, 0.024, 0.047 and 0.026, respectively). Immunoblot analysis revealed that basigin/CD147 expression increased in metastatic SCC.
These results suggest that MMPs produced by both tumor cells and stromal cells play a role in metastasis of cutaneous SCC, and increased MMPs in stromal cells might be induced by basigin/CD147 expressed in tumor cells.
研究基质金属蛋白酶（MMPs）在皮肤鳞状细胞癌（SCC）转移灶中的表达及其与基底膜蛋白/CD147表达的相关性。
采用DAB和ALP免疫组化染色检测14例转移性皮肤鳞状细胞癌中MMPs和基底膜蛋白/CD147的表达，并通过免疫印迹分析观察转移样本中基底膜蛋白/CD147的表达。
正常皮肤和淋巴结中MMPs基本呈阴性，但转移性皮肤鳞状细胞癌的肿瘤细胞和基质细胞中MMP-1、MMP-2、MMP-3和MT1-MMP的表达均增加。转移性皮肤鳞状细胞癌中，分别有4例和8例肿瘤细胞的基底膜蛋白/CD147呈强阳性或阳性染色。肿瘤细胞中基底膜蛋白/CD147的表达与周围基质细胞中MMP-1、MMP-2、MMP-3、MT1-MMP的表达之间存在显著相关性（P值分别为0.012、0.024、0.047和0.着026）。免疫印迹分析显示转移性皮肤鳞状细胞癌中基底膜蛋白/CD147表达增加。
这些结果表明，肿瘤细胞和基质细胞产生的MMPs在皮肤鳞状细胞癌转移中起作用，基质细胞中MMPs的增加可能是由肿瘤细胞中表达的基底膜蛋白/CD147诱导的。
