Hu J Z, Feng D Y, Cheng R X
Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410008, China.
Hunan Yi Ke Da Xue Xue Bao. 2001 Aug 28;26(4):325-7.
To detect the expressions of p-MAPK, cyclin D1 and p53 protein, and investigate their relationship in osteosarcomas.
SP immunohistochemical technique was used to detect the expressions of of p-MAPK, cyclin D1 and p53 protein among the 58 osteosarcomas and 14 osteoid osteomas samples.
The positive rates of p-MAPK, cyclin D1 and p53 protein were 77.6% (45/58), 60.3% (35/58) and 44.8% (26/58) respectively in 58 osteosarcomas, and 21.4% (3/14), 21.4% (3/14) and 7.1% (1/14) in 14 osteoid osteomas. Their positive rates and expressive intensities in osteosarcomas were higher than those in osteiod osteomas. Their positive correlation was significantly observed between p-MAPK and cyclin D1 proteins (P < 0.01), and wasn't observed between p-MAPK and p53 proteins (P < 0.05) in osteosarcomas.
MAPK phosporylation may be one of causes for activation of cyclin D1, which play an important role in osteosarcogenesis. The mutation of p53 oncogene may not result in osteosarcogenesis by MAPK signaling pathway. Detecting p-MAPK by immunohistochemistry may be one of diagnostic indexes for osteosarcoma and benign osteoma.
检测骨肉瘤中p-MAPK、细胞周期蛋白D1及p53蛋白的表达情况，并探讨它们之间的关系。
采用SP免疫组织化学技术检测58例骨肉瘤及14例骨样骨瘤标本中p-MAPK、细胞周期蛋白D1及p53蛋白的表达。
58例骨肉瘤中p-MAPK、细胞周期蛋白D1及p53蛋白的阳性率分别为77.6%（45/58）、60.3%（35/58）和44.8%（26/58），14例骨样骨瘤中分别为21.4%（3/14）、21.4%（3/14）和7.1%（1/14）。骨肉瘤中它们的阳性率及表达强度均高于骨样骨瘤。骨肉瘤中p-MAPK与细胞周期蛋白D1蛋白呈显著正相关（P<0.01），p-MAPK与p53蛋白无相关性（P<0.05）。
MAPK磷酸化可能是细胞周期蛋白D1激活的原因之一，在骨肉瘤发生中起重要作用。p53癌基因突变可能不通过MAPK信号通路导致骨肉瘤发生。免疫组织化学检测p-MAPK可能是骨肉瘤与骨良性肿瘤的诊断指标之一。
