Fu J, Xu Y, Zhang Z
Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
J Tongji Med Univ. 2001;21(4):297-300. doi: 10.1007/BF02886561.
The relations between mRNA expression of basic fibroblast growth factor (bFGF) and the changes in collagen I and collagen III in pulmonary tissues from a single intratracheal instillation of papain-induced emphysema in rats were investigated. Wistar rats (n = 42) were randomly divided into normal group and emphysema model 1, 3, 5, 7, 15, 30-day groups (n = 6 in each group). The rat model of emphysema was induced by a single intratracheal instillation of papain. The results of immunohistochemistry SABC and in situ hybridization with bFGF probe were quantitatively analyzed to examine the changes of collagen I and collagen III and bFGF mRNA expression in lung tissues and the percent of positive expression of bFGFmRNA in alveolar macrophages. The results were as follows: (1) In the emphysema model groups the optical densities of collagen I and collagen III began to increase after 3 days, reached the highest at the 7th day, and began to reduce at the 15th day; (2) No expression of bFGFmRNA in pulmonary tissues was detectable in the normal group. The positive expression of bFGFmRNA was detectable in lung tissues one day after the intratracheal instillation of papain. The average optical densities reached the peak (41.895 +/- 7.017) at the 7th day, significantly higher than in the normal group (0.581 +/- 0.139, P < 0.01). The positive expression of bFGFmRNA in lung tissues began to reduce at the 15th day; (3) Positive expression of bFGFmRNA in alveolar macrophages of instillation papain rats was detectable 3 days after the intratracheal instillation of papain, and reached the highest at the 7th day with the percent of positive expression of bFGF mRNA in alveolar macrophages being 70.13 +/- 11.21, higher than in the normal group (5.12 +/- 0.18, P < 0.01); (4) The expression of bFGF mRNA in the lung tissues and macrophages was positively related with the changes in collagen I and collagen III (P < 0.01 or P < 0.05) respectively. It was suggested that the up-regulation of bFGF mRNA expression during the development of emphysema can lead pulmonary interstitial fibrosis, which may take part in the injury and repair and the lung tissue reconstruction.
研究了大鼠经气管内单次滴注木瓜蛋白酶诱导肺气肿后，肺组织中碱性成纤维细胞生长因子（bFGF）的mRNA表达与I型和III型胶原蛋白变化之间的关系。将42只Wistar大鼠随机分为正常组和肺气肿模型1、3、5、7、15、30天组（每组6只）。通过气管内单次滴注木瓜蛋白酶诱导大鼠肺气肿模型。采用免疫组化SABC法及bFGF探针原位杂交法，定量分析肺组织中I型和III型胶原蛋白及bFGF mRNA表达的变化，以及肺泡巨噬细胞中bFGFmRNA的阳性表达率。结果如下：（1）在肺气肿模型组中，I型和III型胶原蛋白的光密度在3天后开始升高，第7天达到最高，第15天开始下降；（2）正常组肺组织中未检测到bFGFmRNA的表达。气管内滴注木瓜蛋白酶1天后，肺组织中可检测到bFGFmRNA的阳性表达。平均光密度在第7天达到峰值（41.895±7.017），显著高于正常组（0.581±0.139，P<0.01）。肺组织中bFGFmRNA的阳性表达在第15天开始下降；（3）气管内滴注木瓜蛋白酶后3天，可检测到滴注木瓜蛋白酶大鼠肺泡巨噬细胞中bFGFmRNA的阳性表达，第7天达到最高，肺泡巨噬细胞中bFGF mRNA的阳性表达率为70.13±11.21，高于正常组（5.12±0.18，P<0.01）；（4）肺组织和巨噬细胞中bFGF mRNA的表达分别与I型和III型胶原蛋白的变化呈正相关（P<0.01或P<0.05）。提示肺气肿发生发展过程中bFGF mRNA表达上调可导致肺间质纤维化，可能参与肺组织的损伤修复及组织重构。
