Zhen Z C, Yao Z J
Chinese National Human Genome Center, Beijing 100176, China.
Se Pu. 2001 Jul;19(4):361-4.
Depending on the outputs of 10,080 DNA sequencing samples from the human genome project, technical features of capillary array electrophoresis are discussed. The results show that 88% of the total readlength were higher than 50 kbp/run. It means that most of the readlength could reach 500 bp or more with reasonable quality. The results were much better than expected when the technology of capillary array appeared about one year ago. A key parameter affecting the quality of DNA sequencing is the purity of sequencing template. Therefore, a batch agarose slab electrophoresis was applied to check the purity before loading. Other conditions, such as concentration of template and loading amount, are discussed as well. Finally, comparisons based on the electrophoresis timing, the pre-treatment of samples, the loading procedures and the results analysis between capillary array electrophoresis and slab gel electrophoresis have been made. From the point of view of high-throughput DNA sequencing, especially in the field of genome research, the capillary array electrophoresis should be the better choice as a more efficient technical platform.
根据人类基因组计划中10,080个DNA测序样本的输出结果，讨论了毛细管阵列电泳的技术特点。结果表明，总读长的88%高于50 kbp/次运行。这意味着在合理的质量下，大多数读长可以达到500 bp或更长。当毛细管阵列技术大约在一年前出现时，结果比预期的要好得多。影响DNA测序质量的一个关键参数是测序模板的纯度。因此，在加样前应用一批琼脂糖平板电泳来检查纯度。还讨论了其他条件，如模板浓度和加样量。最后，对毛细管阵列电泳和平板凝胶电泳在电泳时间、样品预处理、加样程序和结果分析方面进行了比较。从高通量DNA测序的角度来看，特别是在基因组研究领域，毛细管阵列电泳作为一个更高效的技术平台应该是更好的选择。
