Chambers Stephen P
Vertex Pharmaceuticals, 130 Washington Street, Cambridge, MA 02139, USA.
Drug Discov Today. 2002 Jul 15;7(14):759-65. doi: 10.1016/s1359-6446(02)02361-9.
In the past, protein expression has been perceived as the principle bottleneck in protein characterization and structure determination. The challenge now is to rapidly express large numbers of genes in the search for new drug targets and therapeutic proteins encoded by the human genome. In this competitive environment, several high-throughput expression strategies for protein production are being used to industrialize the process of protein expression.
过去,蛋白质表达被视为蛋白质表征和结构测定的主要瓶颈。如今面临的挑战是在寻找人类基因组编码的新药物靶点和治疗性蛋白质的过程中快速表达大量基因。在这种竞争环境下,几种用于蛋白质生产的高通量表达策略正被用于使蛋白质表达过程工业化。