Qiu J L, Jin Q L, Liu N Z, Wang J
Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032.
Shi Yan Sheng Wu Xue Bao. 1999 Mar;32(1):88-92.
No matter when anion channel inhibitors, DIDS (4, 4'-diisothiocyanatostilbene-2, 2'-disulfonic acid) and A9C (anthracene-9-carboxylic acid) added (before, at the same time of or after harpinPss treatment), they can inhibit harpinPss-induced hypersensitive response in tobacco seedlings and release of active oxygen and extracellular alkalinization in tobacco suspension cells. DIDS and A9C also inhibit harpinPss-induced Ca2+ influx. In all these cases, DIDS is more efficient than A9C. It is postulated that anion channel positively regulates calcium channel in plasma membrane, and harpinPss may function through signal transduction mediated by anion channel and calcium channel to regulate cellular Ca2+ concentration and defense responses.
无论阴离子通道抑制剂DIDS（4,4'-二异硫氰基芪-2,2'-二磺酸）和A9C（蒽-9-羧酸）在何时添加（在harpinPss处理之前、同时或之后），它们都能抑制harpinPss诱导的烟草幼苗过敏反应以及烟草悬浮细胞中活性氧的释放和细胞外碱化。DIDS和A9C还能抑制harpinPss诱导的Ca2+内流。在所有这些情况下，DIDS比A9C更有效。据推测，阴离子通道正向调节质膜中的钙通道，harpinPss可能通过由阴离子通道和钙通道介导的信号转导发挥作用，以调节细胞内Ca2+浓度和防御反应。
