Nan X R, Wei Z M
Biotechnology Research Center, Heilongjiang Academy of Agriculture Science, Harbin 150086.
Shi Yan Sheng Wu Xue Bao. 1999 Dec;32(4):409-13.
The foreign Bt gene was transferred into protoplasts of soybean using PEG and PLO methods, respectively. The result indicated that the transformation frequency of PLO method was about 0.1% higher than PEG method. The PCR and Southern blotting analysis of the regeneration plants confirmed the integration of foreign gene into the genome of soybean.
分别采用聚乙二醇（PEG）法和聚赖氨酸（PLO）法将外源Bt基因导入大豆原生质体。结果表明，PLO法的转化频率比PEG法高约0.1%。对再生植株进行的PCR和Southern杂交分析证实外源基因已整合到大豆基因组中。
