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多重引物聚合酶链反应检测淋病奈瑟菌(NG)、沙眼衣原体(CT)和解脲脲原体(UU)

[Detection of Neisseria gonorrhoeae(NG), Chlamydia trachomatis(CT) and Ureaplasma urealyticum(UU) by multiple primer PCR].

作者信息

Lu Y, Li J, Chen Y

机构信息

Department of Microbiology, Anhui Medical University, Hefei 200036.

出版信息

Wei Sheng Wu Xue Bao. 2000 Apr;40(2):221-3.

Abstract

For the detection of Neisseria gonorrhoeae(NG), Chlamydia trachomat is(CT) and Ureaplasma urealyticum(UU) in urogenital infection in one specimen, multiple primer PCR was used in 156 sex transmitted disease patients, it was shown that in 156 specimens there were 40 NG positive and 31 CT positive and 27 UU positive, 10 NG + CT positive, 4 NG + UU positive, 3 CT + UU positive and 2 NG + CT + UU positive respectively. The results were in correspondence with each other. It is suggested that multiple primer PCR is a quick, sensitive, simple and specific technique which can be applied in clinical test.

摘要

为检测泌尿生殖系统感染患者单一标本中的淋病奈瑟菌(NG)、沙眼衣原体(CT)和解脲脲原体(UU),对156例性传播疾病患者采用多重引物PCR法。结果显示,156份标本中,NG阳性40例,CT阳性31例,UU阳性27例,NG+CT阳性10例,NG+UU阳性4例,CT+UU阳性3例,NG+CT+UU阳性2例。结果相互吻合。提示多重引物PCR是一种快速、灵敏、简便、特异的技术,可应用于临床检测。

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