Sun Y, Han Y, Zhao J F, Bai J, Hao M P, Sun D Y
Department of Biology, Hebei Normal University, Shijiazhuang, 050016.
Shi Yan Sheng Wu Xue Bao. 2000 Sep;33(3):255-62.
The effects of antiserum against human VnR integrin and integrin antagonist GRGDSP peptides on Nicotiana tabacum pollen germination and tube growth both in in vitro and in semi-vivo conditions were studied. No obvious inhibitory effects on pollen germination and tube growth in vitro were observed when anti-VnR serum or GRGDSP peptides was added to BK culture medium, but the enhancement of pollen germination and tube growth in vitro promoted by calmodulin was depressed by adding anti-VnR serum or GRGDSP peptides to BK culture medium. In addition to that, pollen germination and tube growth on stigma, as well as tube growth in styles were also inhibited at some extent by treating stigma and microinjecting GRGDSP peptides or anti-VnR serum into styles. The role of integrin-like proteins in regulation of pollen germination and tube growth in situ was discussed.
研究了抗人VnR整合素抗血清和整合素拮抗剂GRGDSP肽在体外和半体内条件下对烟草花粉萌发和花粉管生长的影响。当将抗VnR血清或GRGDSP肽添加到BK培养基中时，未观察到对体外花粉萌发和花粉管生长有明显的抑制作用，但向BK培养基中添加抗VnR血清或GRGDSP肽会抑制钙调蛋白促进的体外花粉萌发和花粉管生长。除此之外，通过处理柱头以及向花柱中微量注射GRGDSP肽或抗VnR血清，柱头的花粉萌发和花粉管生长以及花柱中的花粉管生长也受到了一定程度的抑制。文中讨论了整合素样蛋白在原位调节花粉萌发和花粉管生长中的作用。
