Xu Z Q, Becker D, Lörz H
Provincial Key Laboratory of Biotechnology, Northwest University, Xian 710069.
Shi Yan Sheng Wu Xue Bao. 2001 Sep;34(3):183-9.
The applicability of hyperosmotic treatment and different configurations of plasmid DNA for stable transformation of wheat mediated by particle bombardment was investigated. Hyperosmotic treatment increased the frequency of transient expression and had also a positive effect on stable transformation. Denaturation of plasmid DNA prior to bombardment led to dramatic reduction of transient expression. However, there were no marked differences between single-stranded and double-stranded DNA in stable transformation. Single-stranded plasmid DNA, double-stranded plasmid DNA in linear state and double-stranded plasmid DNA in circular state could all be used to produce transgenic wheat plants. A total of 26 independent transgenic plants of winter wheat genotype Florida and 4 independent transgenic plants of spring wheat genotype Veery were obtained. Most transgenic plants have set seeds. T2 seeds of some spring wheat transgenic plants have also been harvested.
研究了高渗处理和不同构型的质粒DNA在粒子轰击介导的小麦稳定转化中的适用性。高渗处理提高了瞬时表达频率，对稳定转化也有积极作用。轰击前质粒DNA的变性导致瞬时表达显著降低。然而，单链和双链DNA在稳定转化方面没有显著差异。单链质粒DNA、线性双链质粒DNA和环状双链质粒DNA均可用于培育转基因小麦植株。共获得了26株冬小麦基因型佛罗里达的独立转基因植株和4株春小麦基因型维里的独立转基因植株。大多数转基因植株已结实。一些春小麦转基因植株的T2种子也已收获。
