人精液中的11,15,19 - 三羟基 - 9 - 酮基前列腺 - 13 - 烯酸和11,15,19 - 三羟基 - 9 - 酮基前列腺 - 5,13 - 二烯酸

11, 15, 19-trihydroxy-9-ketoprost-13-enoic acid and 11, 15, 19-trihydroxy-9-ketoprosta-5, 13-dienoic acid in human seminal fluid.

作者信息

Jonsson H T, Middleditch B S, Schexnayder M A, Desiderio D M

出版信息

J Lipid Res. 1976 Jan;17(1):1-6.

Abstract

Two novel prostaglandins (PG) have been found in human seminal fluid which had been frozen immediately after collection. They were characterized by combined gas-liquid chromatography-mass spectrometry of various derivatives as 19-hydroxy prostaglandin E1 (11, 15, 19-trihydroxy-9-ketoprost-13-enoic acid) and 19-hydroxy prostaglandin E2 (11, 15, 19-trihydroxy-9-ketoprosta-5, 13-dienoic acid). They were present in three to five times the quantity of prostaglandins E1 and E2. Incubation of seminal fluid for 3 hr at 25 degrees C reduced levels of 190H-PGEs2.5-fold and PGE22-fold, while increasing levels of PGAs and PGBs 2-fold. No 190H PGA or 190H PGB was detected in extracts of unincubated fluid. The PGAs, PGBs and their 19-hydroxy analogs are probably artifacts arising metabolically or as a result of classical isolation techniques.

摘要

在采集后立即冷冻的人类精液中发现了两种新型前列腺素（PG）。通过对各种衍生物进行气相色谱 - 质谱联用分析，它们被鉴定为19 - 羟基前列腺素E1（11,15,19 - 三羟基 - 9 - 酮基前列腺 - 13 - 烯酸）和19 - 羟基前列腺素E2（11,15,19 - 三羟基 - 9 - 酮基前列腺 - 5,13 - 二烯酸）。它们的含量是前列腺素E1和E2的三到五倍。精液在25℃下孵育3小时，19 - 羟基前列腺素E的水平降低了2.5倍，前列腺素E2降低了2倍，而前列腺素A和前列腺素B的水平增加了2倍。在未孵育的精液提取物中未检测到19 - 羟基前列腺素A或19 - 羟基前列腺素B。前列腺素A、前列腺素B及其19 - 羟基类似物可能是代谢产生的或传统分离技术导致的假象。