人巨细胞病毒主要衣壳蛋白与最小衣壳蛋白之间的相互作用取决于最小衣壳蛋白中的两个线性序列。
The interaction between the major capsid protein and the smallest capsid protein of human cytomegalovirus is dependent on two linear sequences in the smallest capsid protein.
作者信息
Lai Lilin, Britt William J
机构信息
Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35233, USA.
出版信息
J Virol. 2003 Feb;77(4):2730-5. doi: 10.1128/jvi.77.4.2730-2735.2003.
Abstract
The assembly of human cytomegalovirus (HCMV) with recombinant systems has not been accomplished. An understanding of specific interactions between individual capsid proteins could point to unique characteristics of the assembly process of HCMV capsids. Similar to its herpes simplex virus counterpart, VP26 (UL35), the HCMV smallest capsid protein (SCP; UL48/49) decorates hexons in the mature capsid. In contrast to VP26, the HCMV SCP is essential for virus assembly. In this study we have shown that the major capsid protein (MCP) and the SCP interact in the cytoplasm of transfected cells and can be coprecipitated from insect cells expressing the MCP and the SCP. Using a two-hybrid reporter assay, we demonstrated that two linear sequences within the SCP are sufficient for SCP and MCP interactions.
摘要
利用重组系统装配人巨细胞病毒（HCMV）尚未实现。了解单个衣壳蛋白之间的特定相互作用可能有助于揭示HCMV衣壳装配过程的独特特征。与单纯疱疹病毒的对应蛋白VP26（UL35）类似，HCMV最小衣壳蛋白（SCP；UL48/49）修饰成熟衣壳中的六邻体。与VP26不同，HCMV SCP对病毒装配至关重要。在本研究中，我们发现主要衣壳蛋白（MCP）和SCP在转染细胞的细胞质中相互作用，并且可以从表达MCP和SCP的昆虫细胞中共沉淀出来。通过双杂交报告基因分析，我们证明SCP内的两个线性序列足以实现SCP与MCP的相互作用。
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