Gibson W, Clopper K S, Britt W J, Baxter M K
Virology Laboratories, Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
J Virol. 1996 Aug;70(8):5680-3. doi: 10.1128/JVI.70.8.5680-5683.1996.
The capsid of cytomegalovirus contains an abundant, low-molecular-weight protein whose coding sequence within the viral genome had not been identified. We have used a combination of biochemical and immunological techniques to demonstrate that this protein, called the smallest capsid protein in human cytomegalovirus, is encoded by a previously unidentified 225-bp open reading frame (ORF) located between ORFs UL48 and UL49. This short ORF, called UL48/49, is the positional homolog of herpes simplex virus ORF UL35 (encoding capsid protein VP26) and shows partial amino acid sequence identity to positional homologs in human herpes viruses 6 and 7.
巨细胞病毒的衣壳含有一种丰富的低分子量蛋白质，其在病毒基因组中的编码序列尚未被鉴定。我们使用生物化学和免疫学技术相结合的方法来证明，这种被称为人巨细胞病毒中最小衣壳蛋白的蛋白质，由位于开放阅读框（ORF）UL48和UL49之间一个先前未被鉴定的225碱基对的开放阅读框编码。这个短的开放阅读框，称为UL48/49，是单纯疱疹病毒开放阅读框UL35（编码衣壳蛋白VP26）的位置同源物，并且与人疱疹病毒6型和7型中的位置同源物显示出部分氨基酸序列同一性。
