高效液相色谱法（HPLC）荧光检测法测定葡萄酒中的总亚硫酸盐

[Determination of total sulfite in wine by high performance liquid chromatography(HPLC) with fluorescence detection].

作者信息

Wei F, Chen M S, Yu L, Chang L, Li D M

机构信息

Dalian Import Food Hygiene Supervision and Inspection Center of P.R.C., Dalian 116001, China.

出版信息

Se Pu. 1999 Nov;17(6):583-5.

PMID:12552699

Abstract

A highly sensitive fluorometric method for the determination of total sulfite in wine is reported. The strong fluorescent derivatives formed by the quantitative reaction of sulfite in wine and N-(9-acridinyl) maleimide(NAM) in aqueous solution under mild conditions was injected into the HPLC. Separation was performed on an ODS column(200 mm x 5 mm) with a mobile phase of V(CH3CN):V(0.1 mo/L NH4AC) = 25:75 at a flow rate of 1.0 mL/min and detected by fluorometry at 455 nm(excitation at 365 nm). There was a good linear calibration curve in the range from 0.25-4 mg/L(r = 0.9998), The limit of detection was 1 ng. Sulfite mass concentration in wine has been determined by this method with good results and recoveries. This method is simple, rapid and reliable, and a sample size of only 1 mL is required to complete an analysis.

摘要

报道了一种用于测定葡萄酒中总亚硫酸盐的高灵敏度荧光法。葡萄酒中的亚硫酸盐与N-（9-吖啶基）马来酰亚胺（NAM）在温和条件下于水溶液中定量反应形成的强荧光衍生物被注入高效液相色谱仪。在ODS柱（200 mm×5 mm）上进行分离，流动相为V（乙腈）：V（0.1 mo/L NH₄AC）=25:75，流速为1.0 mL/min，并通过荧光法在455 nm处检测（激发波长为365 nm）。在0.25 - 4 mg/L范围内有良好的线性校准曲线（r = 0.9998），检测限为1 ng。用该方法测定了葡萄酒中亚硫酸盐的质量浓度，结果和回收率良好。该方法简单、快速且可靠，完成一次分析仅需1 mL样品量。