[毛细管高效液相色谱法检测蜂蜜中残留抗生素]
[Detection of residual antibiotics in honey by capillary high performance liquid chromatography].
作者信息
Huang H N, Chen T B, Chen R M, Rao P F
机构信息
Institute of Biotechnology, Fuzhou University, Fuzhou 350002, China.
出版信息
Se Pu. 1999 Nov;17(6):588-9.
The quantitative analysis of the three antibiotics-tetracycline(TC), oxytetracycline(OTC) and chlortetracyclinum(CTC) was carried out with a self-packed Hypersil C18 150 mm x 0.5 mm i.d. column. As little as 0.5 nanogram of antibiotics can be well detected, the capillary high performance liquid chromatographic method is 100 times more sensitive than that with ordinary HPLC method. The coefficients of linear correlation of mass concentration from 0.5 nanogram to 20 nanogram were rOTC = 0.99695, rTC = 0.99778 and rOTC = 0.98836. It showed that capillary HPLC is a good method used for sensitive analysis of antibiotics in honey.
采用自装的内径为0.5mm、长150mm的Hypersil C18柱对三种抗生素——四环素（TC）、土霉素（OTC）和金霉素（CTC）进行了定量分析。抗生素的检测限低至0.5纳克，毛细管高效液相色谱法的灵敏度比普通高效液相色谱法高100倍。质量浓度在0.5纳克至20纳克之间的线性相关系数分别为：rOTC = 0.99695，rTC = 0.99778，rCTC = 0.98836。结果表明，毛细管高效液相色谱法是一种用于蜂蜜中抗生素灵敏分析的良好方法。
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