抗生素化学分析的改进。二十三。用电喷雾高效液相色谱-串联质谱法鉴定牛组织中的残留四环素。

Improvement of chemical analysis of antibiotics. XXIII. Identification of residual tetracyclines in bovine tissues by electrospray high-performance liquid chromatography-tandem mass spectrometry.

作者信息

Oka H, Ikai Y, Ito Y, Hayakawa J, Harada K, Suzuki M, Odani H, Maeda K

机构信息

Aichi Prefectural Institute of Public Health, Kita-ku, Nagoya, Japan.

出版信息

J Chromatogr B Biomed Sci Appl. 1997 Jun 6;693(2):337-44.

PMID:9210437

Abstract

To reliably identify the residual tetracycline antibiotics (TCs), oxytetracycline (OTC), tetracycline, chlortetracycline (CTC) and doxycycline (DC), in bovine tissues, we have established a confirmation method using electrospray ionization liquid chromatography-tandem mass spectrometry (ESI LC-MS-MS) with daughter ion scan. All TCs gave [M+H-NH3]+ and [M+H-NH3-H2O]+ as the product ions, except for DC when [M+H]+ was selected as the precursor ion. The combination of C18 cartridge clean-up and the present ESI LC-MS-MS method can reliably identify TCs fortified at a concentration of 0.1 ppm in bovine tissues, including liver, kidney and muscle, and has been successfully applied to the identification of residual OTC in bovine liver and residual CTC in bovine muscle samples previously found at concentrations of 0.58 ppm and 0.38 ppm by LC, respectively.

摘要

为了可靠地鉴定牛组织中的残留四环素类抗生素（TCs），即土霉素（OTC）、四环素、金霉素（CTC）和强力霉素（DC），我们建立了一种使用电喷雾电离液相色谱 - 串联质谱法（ESI LC-MS-MS）并进行子离子扫描的确认方法。除了DC选择[M + H]+作为母离子外，所有四环素类抗生素均产生[M + H - NH3]+和[M + H - NH3 - H2O]+作为产物离子。C18柱净化与当前的ESI LC-MS-MS方法相结合，能够可靠地鉴定牛组织（包括肝脏、肾脏和肌肉）中浓度为0.1 ppm的强化四环素类抗生素，并且已成功应用于鉴定牛肝脏中的残留OTC以及牛肌肉样品中的残留CTC，此前通过液相色谱法分别检测到其浓度为0.58 ppm和0.38 ppm。