Yu H, Shi Y, Zhang Y, Yang S, Shen Z
Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, Beijing 100084, China.
Wei Sheng Wu Xue Bao. 2001 Oct;41(5):548-52.
Vitreoscilla hemoglobin gene (vgb) was introduced into the recombinant Escherichia coli VG1(pTU14) for production of poly-beta-hydroxybutyrate (PHB), in order to enhance the oxygen uptake of strain from molecular level and to solve the problem of oxygen limitation in process of fermentation. The carbon monoxide difference spectra analysis of Vitreoscilla hemoglobin (VHb) showed that vgb could be successfully expressed in VG1 (pTU14), and this expression was under the regulation of dissolved oxygen (DO). The higher amounts of VHb could be got correspond to the lower level of DO. Because of the introduction of vgb, cell growth and PHB accumulation of VG1 (pTU14) were all strongly promoted during the process of batch culture.
将透明颤菌血红蛋白基因（vgb）导入重组大肠杆菌VG1(pTU14) 用于聚-β-羟基丁酸酯（PHB）的生产，目的是从分子水平提高菌株的氧摄取量，并解决发酵过程中的氧限制问题。对透明颤菌血红蛋白（VHb）的一氧化碳差光谱分析表明，vgb能够在VG1 (pTU14) 中成功表达，且这种表达受溶解氧（DO）的调控。溶解氧水平越低，获得的VHb量越高。由于vgb的导入，在分批培养过程中，VG1 (pTU14) 的细胞生长和PHB积累均得到显著促进。
