Yu Huimin, Shi Yue, Zhang Yanping, Yang Shengli, Shen Zhongyao
Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, Beijing 100084, PR China.
FEMS Microbiol Lett. 2002 Sep 10;214(2):223-7. doi: 10.1111/j.1574-6968.2002.tb11351.x.
In order to attain high cell density and low cost production of poly(beta-hydroxybutyrate) (PHB), the Vitreoscilla globin gene (vgb) was introduced into a novel recombinant strain, Escherichia coli VG1 (pTU14). Experiments showed that the expression of vgb was under the regulation of dissolved oxygen (DO) in broth and the introduction of vgb in VG1 (pTU14) induced the parent promotion effect on cell growth and PHB accumulation, especially under low DO conditions. Further experiments indicated that the introduction of vgb in VG1 (pTU14) not only decreased the critical oxygen concentration, but also affected the volumetric oxygen transfer coefficient of the recombinant strain.
为了实现聚（β-羟基丁酸酯）（PHB）的高细胞密度和低成本生产，将透明颤菌血红蛋白基因（vgb）导入一种新型重组菌株大肠杆菌VG1（pTU14）。实验表明，vgb的表达受发酵液中溶解氧（DO）的调节，并且在VG1（pTU14）中引入vgb诱导了对细胞生长和PHB积累的亲本促进作用，特别是在低溶解氧条件下。进一步的实验表明，在VG1（pTU14）中引入vgb不仅降低了临界氧浓度，还影响了重组菌株的体积氧传递系数。
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