[里氏木霉EG I的3'-非翻译区对其在酿酒酵母中基因表达的影响]
[Effect of 3'-UTR of EG I from Trichoderma reesei on its gene expression in Saccharomyces cerevisiae].
作者信息
Xiao Z, Wu Z, Wang T, Qu Y, Gao P, Wang T
机构信息
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
出版信息
Wei Sheng Wu Xue Bao. 2001 Oct;41(5):587-91.
Several industrial yeast are developed as ideal expression hosts for the production of the commercially useful proteins. The expression levels in yeast cells of the heterologous proteins are affected by the regulation factors of the genes themselves. The full-length cDNA coding for EG I from Trichoderma reesei, the cellulose-degrading filamentous fungus, was expressed in Saccharomyces cerevisiae H158. EG I produced by the recombinant S. cerevisiae exhibits maximal activity at 50 degrees C-60 degrees C, pH 5.0. It was observed that removal of the 3'-untranslated region (3'-UTR) from EG I cDNA resulted in no active EG I produced by recombinant yeast. RT-PCR analysis indicated that unlike the yeast cells harboring full-length EG I cDNA, there was no detectable EG I mRNA in the yeast cells harboring EG I cDNA without 3'-UTR. The data suggested that 3'-UTR is important for the expression of EG I in Saccharomyces cerevisiae.
几种工业酵母被开发为生产商业上有用蛋白质的理想表达宿主。异源蛋白质在酵母细胞中的表达水平受基因自身调控因子的影响。编码来自纤维素降解丝状真菌里氏木霉的内切葡聚糖酶I（EG I）的全长cDNA在酿酒酵母H158中表达。重组酿酒酵母产生的EG I在50℃-60℃、pH 5.0时表现出最大活性。据观察，从EG I cDNA中去除3'-非翻译区（3'-UTR）会导致重组酵母不产生有活性的EG I。逆转录聚合酶链反应（RT-PCR）分析表明，与携带全长EG I cDNA的酵母细胞不同，在携带不含3'-UTR的EG I cDNA的酵母细胞中未检测到EG I mRNA。数据表明3'-UTR对EG I在酿酒酵母中的表达很重要。
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