Long Y, Yang X, Huang T
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, 610041.
Se Pu. 1999 Jul;17(4):339-41.
A novel bonded stationary phase, tetradecylamine bonded stationary phase (TABP), for reversed-phase HPLC was prepared by bonding 1-tetradecylamine to YWG-80 silica gel through 3-glycidoxypropyltrimethoxysilane. Hydrophobicity, selectivity and silanophilic activity of TABP were evaluated by using aromatic compounds as analytes and methanol-water as binary mobile phase. The orgainc components including acidic, basic and neutral aromatic analytes could be separated satisfactorily with excellent selectivity and peak shape. The relative retention alpha for ethylbenzene-toluene was found to be 1.66 and the asymmetry factors of basic aniline, p-toluidine and N,N-dimethylaniline were found to be 1.26, 1.21 and 1.10, respectively, with the V(methanol):V(water) = 55:45 mobile phase. Aniline was eluted before phenol due to the suppress of the ion exchange activity of residual silanols by the internal masking interaction.
通过3-缩水甘油氧基丙基三甲氧基硅烷将十四烷基胺键合到YWG-80硅胶上，制备了一种用于反相高效液相色谱的新型键合固定相——十四烷基胺键合固定相（TABP）。以芳香族化合物为分析物，甲醇-水为二元流动相，对TABP的疏水性、选择性和硅醇基活性进行了评价。包括酸性、碱性和中性芳香族分析物在内的有机成分能够以优异的选择性和峰形得到满意的分离。在V（甲醇）:V（水）=55:45的流动相条件下，乙苯-甲苯的相对保留值α为1.66，碱性苯胺、对甲苯胺和N,N-二甲基苯胺的不对称因子分别为1.26、1.21和1.10。由于内部掩蔽作用抑制了残留硅醇基的离子交换活性，苯胺比苯酚先被洗脱。
