[一种通过粪便抗原检测来检测和根除幽门螺杆菌感染的新方法]

[A new method for detection and erradication of Helicobacter pylori infection by stool antigens test].

作者信息

Amèndola R, Doweck J, Katz J, Racca J, Menendez G, Schenone L, Farìas R, Barrantes C, Quintanta C, Zerbo O, Kogan Z, Valero J, Bartellini M A, Questa U, Luna P, Corti R E

机构信息

Servicio de Laboratorio, Endoscopìa, Unidad Esòfago y Estòmago, Hospital de Gastroenterologia Dr. Carlos Bonorino Udaondo, Buenos Aires, Argentina.

出版信息

Acta Gastroenterol Latinoam. 2002;32(2):83-5.

PMID:12553159

Abstract

Nowadays technics for Helicobacter pylori detection in stools like culture, and PCR, are expensive and difficult to perform. The aim of this study was to evaluate ELISA test efficacy for detection of H. Pylori antigens in stools comparing this results with standarized technics like histology (Giemsa), ureasa test and UBT C 14. 26 patients were evaluated in this study, ages between 15-75 with upper gastrointestinal symptoms; all of them required gastroduodenal endoscopy, status H. Pylori was determined with methods upon mentioned. 24 hours after endoscopy H. Pylori antigens in stools with the technique Premier Platinum Htsa, Elisa were determined. The detection of H. Pylori antigens in stools accurately identified active H. Pylori infection. The performance characteristics of this non-invasive method was similar in sensibility and specificity to conventional tests.

摘要

如今，用于检测粪便中幽门螺杆菌的技术，如培养和聚合酶链反应（PCR），成本高昂且操作困难。本研究的目的是评估酶联免疫吸附测定（ELISA）检测粪便中幽门螺杆菌抗原的效果，并将该结果与组织学（吉姆萨染色）、尿素酶试验和碳-14尿素呼气试验（UBT C 14）等标准化技术进行比较。本研究评估了26例患者，年龄在15至75岁之间，均有上消化道症状；他们都需要进行胃十二指肠内镜检查，采用上述方法确定幽门螺杆菌感染状况。内镜检查24小时后，采用普瑞米尔铂Htsa酶联免疫吸附测定技术检测粪便中的幽门螺杆菌抗原。粪便中幽门螺杆菌抗原的检测准确地识别了活动性幽门螺杆菌感染。这种非侵入性方法的性能特征在敏感性和特异性方面与传统检测方法相似。