Aksoy Duygu Y, Aybar Melda, Ozaslan Ersan, Kav Taylan, Engin Doruk, Ercis Serpil, Altinok Gulcin, Hascelik Gulsen, Uzunalimoglu Bedri, Arslan Serap
Department of Internal Medicine, Section of Gastroenterology, Hacettepe University, Ankara, Turkey.
Hepatogastroenterology. 2003 Jul-Aug;50(52):1047-9.
BACKGROUND/AIMS: Several invasive and non-invasive methods are available for the detection of H. pylori infection. The accuracy of anti-H. pylori antibodies in serum is low. There is a need for a quick, inexpensive and reliable non-invasive test to detect H. pylori. The aim of this study was to evaluate the enzyme immunoassay for the detection of H. pylori antigen in stool in the Turkish population and compare it to other methods.
50 patients who were admitted to Hacettepe University Department of Internal Medicine, Division of Gastroenterology with the symptom of dyspepsia for whom the indication of upper gastrointestinal endoscopy was present were included in the study. With their permission stool samples were taken. The patients were evaluated with histology, culture, serology, rapid urease test and HpSA (Helicobacter pylori Stool Antigen test). Forty-one patients had gastritis and biopsies were taken from those.
Excluding HpSA if three of the rest of four methods were positive, patients were accepted as H. pylori positive. Nineteen patients were positive for H. pylori, 22 were negative. HpSA was positive in 16 of 19. The sensitivity and specificity of the methods were as follows: histology 100% sensitive, and 86% specific, culture 63% and 100%, HpIgG 58% and 73%, rapid urease test 89% and 82%, respectively. The results were as 84% and 82% for HpSA. Comparing with the 'Gold Standard' histology using McNemar's test Kappa results were as 0.610, 0.181, 0.610, 0.708 for culture, HpIgG, Rapid Urease Test and HpSA, respectively.
HpSA is a cheap, effective method for the diagnosis of H. pylori infection in the Turkish population.
背景/目的：有多种侵入性和非侵入性方法可用于检测幽门螺杆菌感染。血清中抗幽门螺杆菌抗体的准确性较低。需要一种快速、廉价且可靠的非侵入性检测方法来检测幽门螺杆菌。本研究的目的是评估酶免疫分析法在土耳其人群中检测粪便中幽门螺杆菌抗原的效果，并将其与其他方法进行比较。
纳入50例因消化不良症状入住哈杰泰佩大学内科胃肠病学教研室且有上消化道内镜检查指征的患者。经他们同意后采集粪便样本。对患者进行组织学、培养、血清学、快速尿素酶试验和HpSA（幽门螺杆菌粪便抗原检测）评估。41例患者患有胃炎，并对其进行了活检。
排除HpSA，如果其余四种方法中的三种呈阳性，则患者被判定为幽门螺杆菌阳性。19例患者幽门螺杆菌阳性，22例阴性。19例中有16例HpSA呈阳性。各方法的敏感性和特异性如下：组织学分别为100%敏感和86%特异，培养为63%和100%，HpIgG为58%和73%，快速尿素酶试验为89%和82%。HpSA的结果分别为84%和82%。使用McNemar检验与“金标准”组织学比较，培养、HpIgG、快速尿素酶试验和HpSA的Kappa结果分别为0.610、0.181、0.610、0.708。
HpSA是土耳其人群中诊断幽门螺杆菌感染的一种廉价、有效的方法。
