Cruze C A, Meyer M C
J Pharm Sci. 1976 Jan;65(1):33-7. doi: 10.1002/jps.2600650105.
The binding of salicylic acid and sulfathiazole to bovine whole blood, plasma proteins, and purified albumin fraction was investigated using a dynamic dialysis system. The binding profiles for salicylic acid were quite similar in bovine plasma and 4% bovine serum albumin. In contrast, the binding of sulfathiazole was significantly greater in the plasma than in solutions of fraction V bovine serum albumin. Data from dynamic dialysis binding studies of the compounds, conducted in whole blood and suspended erythrocyte systems, did not lend themselves to analysis by classical methods. Hemolysis and alteration in the nature of the protein binding sites during the binding studies were shown to be factors that could explain the unusual binding observed in the whole blood system.
使用动态透析系统研究了水杨酸和磺胺噻唑与牛全血、血浆蛋白及纯化白蛋白组分的结合情况。水杨酸在牛血浆和4%牛血清白蛋白中的结合曲线相当相似。相比之下，磺胺噻唑在血浆中的结合明显高于在V组分牛血清白蛋白溶液中的结合。在全血和悬浮红细胞系统中进行的化合物动态透析结合研究数据，无法用经典方法进行分析。结合研究过程中的溶血现象以及蛋白质结合位点性质的改变被证明是可以解释在全血系统中观察到的异常结合的因素。
