组蛋白H1增强了染色质中MMTV启动子的协同激活作用。
Histone H1 enhances synergistic activation of the MMTV promoter in chromatin.
作者信息
Koop Ronald, Di Croce Luciano, Beato Miguel
机构信息
Institut für Molekularbiologie und Tumorforschung (IMT), Philipps-Universität, E.-Mannkopff-Strasse 2, D-35033 Marburg, Germany.
出版信息
EMBO J. 2003 Feb 3;22(3):588-99. doi: 10.1093/emboj/cdg052.
Abstract
Minichromosomes assembled on the mouse mammary tumor virus (MMTV) promoter in vitro exhibit positioned nucleosomes, one of which covers the binding sites for progesterone receptor (PR) and nuclear factor 1 (NF1). Incorporation of histone H1 into MMTV minichromosomes improves the stability of this nucleosome and decreases basal transcription from the MMTV promoter, as well as its response to either PR or NF1. However, histone H1-containing minichromosomes display better PR binding and support a more efficient synergism between PR and NF1, leading to enhanced transcription initiation. A mutant MMTV promoter lacking positioned nucleosomes does not display enhanced transcriptional synergism in the presence of H1. Binding of PR leads to phosphorylation of H1, which leaves the promoter upon transcription initiation. Thus, H1 assumes a complex and dynamic role in the regulation of the MMTV promoter.
摘要
在体外组装于小鼠乳腺肿瘤病毒（MMTV）启动子上的微型染色体呈现出定位核小体，其中一个核小体覆盖了孕激素受体（PR）和核因子1（NF1）的结合位点。将组蛋白H1掺入MMTV微型染色体可提高该核小体的稳定性，并降低MMTV启动子的基础转录及其对PR或NF1的反应。然而，含有组蛋白H1的微型染色体显示出更好的PR结合，并支持PR与NF1之间更有效的协同作用，从而导致转录起始增强。缺乏定位核小体的突变型MMTV启动子在存在H1的情况下不会显示出增强的转录协同作用。PR的结合导致H1磷酸化，其在转录起始时离开启动子。因此，H1在MMTV启动子的调控中发挥着复杂而动态的作用。
相似文献
1
Histone H1 enhances synergistic activation of the MMTV promoter in chromatin.组蛋白H1增强了染色质中MMTV启动子的协同激活作用。
EMBO J. 2003 Feb 3;22(3):588-99. doi: 10.1093/emboj/cdg052.
2
The mouse mammary tumour virus promoter positioned on a tetramer of histones H3 and H4 binds nuclear factor 1 and OTF1.位于组蛋白H3和H4四聚体上的小鼠乳腺肿瘤病毒启动子与核因子1和OTF1结合。
J Mol Biol. 1998 May 15;278(4):725-39. doi: 10.1006/jmbi.1998.1718.
3
4
Histone acetylation characterizes chromatin presetting by NF1 and Oct1 and enhances glucocorticoid receptor binding to the MMTV promoter.组蛋白乙酰化是NF1和Oct1对染色质进行预设的特征，并增强糖皮质激素受体与MMTV启动子的结合。
Exp Cell Res. 2009 Sep 10;315(15):2604-15. doi: 10.1016/j.yexcr.2009.05.012. Epub 2009 May 20.
5
Complex role of histone H1 in transactivation of MMTV promoter chromatin by progesterone receptor.组蛋白H1在孕激素受体对MMTV启动子染色质的反式激活中的复杂作用。
J Steroid Biochem Mol Biol. 2002 Dec;83(1-5):15-23. doi: 10.1016/s0960-0760(02)00253-4.
6
Visualization of multicomponent transcription factor complexes on chromatin and nonnucleosomal templates in vivo.体内染色质和非核小体模板上多组分转录因子复合物的可视化。
Methods. 1997 Feb;11(2):235-45. doi: 10.1006/meth.1996.0410.
7
FoxA1 binding to the MMTV LTR modulates chromatin structure and transcription.FoxA1与MMTV长末端重复序列的结合调节染色质结构和转录。
Exp Cell Res. 2005 Apr 1;304(2):593-603. doi: 10.1016/j.yexcr.2004.12.002. Epub 2004 Dec 30.
8
Transcriptional silencing of the mouse mammary tumor virus promoter through chromatin remodeling is concomitant with histone H1 phosphorylation and histone H3 hyperphosphorylation at M phase.通过染色质重塑对小鼠乳腺肿瘤病毒启动子进行转录沉默与M期组蛋白H1磷酸化和组蛋白H3过度磷酸化同时发生。
Virology. 2006 Mar 1;346(1):1-6. doi: 10.1016/j.virol.2005.12.034. Epub 2006 Feb 3.
9
Assembly of MMTV promoter minichromosomes with positioned nucleosomes precludes NF1 access but not restriction enzyme cleavage.具有定位核小体的MMTV启动子微型染色体组装可阻止NF1进入，但不影响限制酶切割。
Nucleic Acids Res. 1998 Aug 15;26(16):3657-66. doi: 10.1093/nar/26.16.3657.
10
Glucocorticoid hormone-induced receptor localization to the chromatin fibers formed on injected DNA in Xenopus oocytes.糖皮质激素诱导的受体定位于非洲爪蟾卵母细胞中注射DNA上形成的染色质纤维。
Exp Cell Res. 2001 May 1;265(2):319-28. doi: 10.1006/excr.2001.5184.
引用本文的文献
1
Phosphorylation-dependent association of human chromatin protein PC4 to linker histone H1 regulates genome organization and transcription.磷酸化依赖的人染色质蛋白 PC4 与连接组蛋白 H1 的关联调节基因组组织和转录。
Nucleic Acids Res. 2022 Jun 24;50(11):6116-6136. doi: 10.1093/nar/gkac450.
2
Linker histone variant HIS1-3 and WRKY1 oppositely regulate salt stress tolerance in Arabidopsis.组蛋白变体 HIS1-3 和 WRKY1 分别正向和负向调控拟南芥的耐盐性。
Plant Physiol. 2022 Jun 27;189(3):1833-1847. doi: 10.1093/plphys/kiac174.
3
Epigenetic Modifications in Tumor-Associated Macrophages: A New Perspective for an Old Foe.肿瘤相关巨噬细胞中的表观遗传修饰：老对手的新视角。
Front Immunol. 2022 Jan 24;13:836223. doi: 10.3389/fimmu.2022.836223. eCollection 2022.
4
Histone H1 Post-Translational Modifications: Update and Future Perspectives.组蛋白 H1 的翻译后修饰：更新与未来展望。
Int J Mol Sci. 2020 Aug 18;21(16):5941. doi: 10.3390/ijms21165941.
5
90 YEARS OF PROGESTERONE: Molecular mechanisms of progesterone receptor action on the breast cancer genome.90 年的孕激素：孕激素受体在乳腺癌基因组上作用的分子机制。
J Mol Endocrinol. 2020 Jul;65(1):T65-T79. doi: 10.1530/JME-19-0266.
6
The embryonic linker histone dBigH1 alters the functional state of active chromatin.胚胎连接组蛋白 dBigH1 改变了活性染色质的功能状态。
Nucleic Acids Res. 2020 May 7;48(8):4147-4160. doi: 10.1093/nar/gkaa122.
7
The High Mobility Group A1 (HMGA1) Chromatin Architectural Factor Modulates Nuclear Stiffness in Breast Cancer Cells.高迁移率族 A1（HMGA1）染色质结构因子调节乳腺癌细胞的核硬度。
Int J Mol Sci. 2019 Jun 4;20(11):2733. doi: 10.3390/ijms20112733.
8
The H1 linker histones: multifunctional proteins beyond the nucleosomal core particle.H1连接组蛋白：核小体核心颗粒之外的多功能蛋白质。
EMBO Rep. 2015 Nov;16(11):1439-53. doi: 10.15252/embr.201540749. Epub 2015 Oct 15.
9
Silencing of IFN-stimulated gene transcription is regulated by histone H1 and its chaperone TAF-I.干扰素刺激基因转录的沉默受组蛋白H1及其伴侣蛋白TAF-I调控。
Nucleic Acids Res. 2014 Jul;42(12):7642-53. doi: 10.1093/nar/gku485. Epub 2014 May 30.
10
Pin1 promotes histone H1 dephosphorylation and stabilizes its binding to chromatin.Pin1 促进组蛋白 H1 的去磷酸化，并稳定其与染色质的结合。
J Cell Biol. 2013 Oct 14;203(1):57-71. doi: 10.1083/jcb.201305159. Epub 2013 Oct 7.
本文引用的文献
1
Asymmetric binding of histone H1 stabilizes MMTV nucleosomes and the interaction of progesterone receptor with the exposed HRE.组蛋白H1的不对称结合稳定了小鼠乳腺肿瘤病毒核小体以及孕激素受体与暴露的激素反应元件之间的相互作用。
J Mol Biol. 2002 Nov 29;324(3):501-17. doi: 10.1016/s0022-2836(02)01101-4.
2
Histone H1 represses estrogen receptor alpha transcriptional activity by selectively inhibiting receptor-mediated transcription initiation.组蛋白H1通过选择性抑制受体介导的转录起始来抑制雌激素受体α的转录活性。
Mol Cell Biol. 2002 Apr;22(8):2463-71. doi: 10.1128/MCB.22.8.2463-2471.2002.
3
Phosphorylation of linker histones regulates ATP-dependent chromatin remodeling enzymes.连接组蛋白的磷酸化调节依赖ATP的染色质重塑酶。
Nat Struct Biol. 2002 Apr;9(4):263-7. doi: 10.1038/nsb776.
4
Accurate chromatin organization of the mouse mammary tumor virus promoter determines the nature of the synergism between transcription factors.小鼠乳腺肿瘤病毒启动子精确的染色质组织决定了转录因子之间协同作用的性质。
J Biol Chem. 2002 Feb 15;277(7):4911-7. doi: 10.1074/jbc.M110094200. Epub 2001 Nov 30.
5
Hormone-mediated dephosphorylation of specific histone H1 isoforms.激素介导的特定组蛋白H1亚型的去磷酸化作用。
J Biol Chem. 2001 Sep 28;276(39):36467-73. doi: 10.1074/jbc.M104641200. Epub 2001 Jul 30.
6
A compendium of the histone H1 family of somatic subtypes: an elusive cast of characters and their characteristics.体细胞亚型组蛋白H1家族简编：一群难以捉摸的角色及其特征
Biochem Cell Biol. 2001;79(3):289-304.
7
Histone H1 phosphorylation by Cdk2 selectively modulates mouse mammary tumor virus transcription through chromatin remodeling.细胞周期蛋白依赖性激酶2介导的组蛋白H1磷酸化通过染色质重塑选择性地调节小鼠乳腺肿瘤病毒转录。
Mol Cell Biol. 2001 Aug;21(16):5417-25. doi: 10.1128/MCB.21.16.5417-5425.2001.
8
Histone H1 diversity: bridging regulatory signals to linker histone function.组蛋白H1多样性：连接调控信号与连接组蛋白功能
Gene. 2001 Jun 13;271(1):1-12. doi: 10.1016/s0378-1119(01)00495-4.
9
Rapid exchange of histone H1.1 on chromatin in living human cells.活的人类细胞中染色质上组蛋白H1.1的快速交换。
Nature. 2000 Dec 14;408(6814):873-6. doi: 10.1038/35048603.
10
Human SWI/SNF nucleosome remodeling activity is partially inhibited by linker histone H1.人源SWI/SNF核小体重塑活性受到连接组蛋白H1的部分抑制。
Biochemistry. 2000 Sep 26;39(38):11649-56. doi: 10.1021/bi001330z.
Zotero 插件