Szép Szilvia, Wang Jimin, Moore Peter B
Department of Chemistry, Yale University, New Haven, Connecticut 06520, USA.
RNA. 2003 Jan;9(1):44-51. doi: 10.1261/rna.2107303.
A crystal structure has been obtained for a 26-nucleotide RNA that contains the loop E sequence from Chromatium minutissimum. Rather than having a loop E-like conformation, it consists of an A-form helix that splits into two separate strands following a sheared A-G base pair. The backbone of the strand containing the G of the A-G pair makes a turn of almost 180 degrees in the space of two nucleotides, and then interacts with the minor groove of the helix from which it originates. Similar structures, which we call hook-turns, occur in 16S and 23S rRNAs. They are found at places where the two strands of a helix separate at an A/G juxtaposition to interact with other sequences.
已获得一种含有微小色菌环E序列的26个核苷酸的RNA的晶体结构。它并非具有类似环E的构象，而是由一个A-型螺旋组成，该螺旋在一个剪切的A-G碱基对后分裂成两条单独的链。包含A-G对中G的链的主链在两个核苷酸的空间内旋转近180度，然后与它起源的螺旋的小沟相互作用。类似的结构，我们称之为钩形转角，出现在16S和23S rRNA中。它们出现在螺旋的两条链在A/G并列处分开以与其他序列相互作用的地方。
