Wang Z, Zhuge J, Fang H
Research and Design Center of Fermentation Glycerol, Wuxi University of Light Industry, Wuxi 214036.
Wei Sheng Wu Xue Bao. 1999 Feb;39(1):68-74.
The strain WL2002-5 isolated from natural samples and overproduced glycerol from glucose was identified systematically. The WL2002-5 fermented glucose and weakly fermented sucrose; assimilated glucose, sucrose, ethanol, weakly assimilated glycerol and citrate, did not assimilated nitrate, erythritol, arabinitol, mannitol. It grew well on the slopes containing 500 g/L of glucose or 10 mL/L acetate or at the temperature up to 40 degrees C. The minimum water activity for its growth was 0.890. It had a negative reaction with DBB and did vegetative reproduction by budding and easily formed Candida-type pseudohyphae but had no sexual reproduction. The molecular weight of its mitochondrial DNA was 20 kb. We conclude that WL2002-5 is a new species of Candida and nominated it Candida glycerolgenesis Zhuge sp. nov..
对从自然样品中分离得到的、能从葡萄糖过量生产甘油的菌株WL2002-5进行了系统鉴定。WL2002-5能发酵葡萄糖且能微弱发酵蔗糖；能同化葡萄糖、蔗糖、乙醇，能微弱同化甘油和柠檬酸盐，不能同化硝酸盐、赤藓糖醇、阿拉伯糖醇、甘露醇。它在含500 g/L葡萄糖或10 mL/L乙酸盐的培养基斜面上生长良好，在高达40℃的温度下也能生长。其生长的最低水分活度为0.890。它对DBB呈阴性反应，通过出芽进行营养繁殖，容易形成假丝酵母型假菌丝，但无有性繁殖。其线粒体DNA的分子量为20 kb。我们得出结论，WL2002-5是假丝酵母属的一个新种，并将其命名为产甘油假丝酵母诸葛新种（Candida glycerolgenesis Zhuge sp. nov.）。
