Xu H, Ouyang P, Zhou W
Department of Biotechnology and Bioengineering, Nanjing Institute of Chemical Technology, Nanjing 210009.
Wei Sheng Wu Xue Bao. 1999 Jun;39(3):272-4.
E. coli EP8-10 was selected from the soil. It was able to produce the transaminase with high activity when it was cultivated on the medium containing peptone and beef extract. Optimum conditions of enzyme reaction was: phenylpyruvic acid's concentration of 0.3-0.5 mol/L, L-Asptaric acid used as amino donor, pH 8.5 37 degrees C. When phenylpyruvic acid was 0.3 mol/L, 48 g/L L-phenylalanine was produced after 6 h with 97% conversion rate.
大肠杆菌EP8 - 10是从土壤中筛选出来的。当它在含有蛋白胨和牛肉膏的培养基上培养时，能够产生高活性的转氨酶。酶反应的最佳条件为：苯丙酮酸浓度0.3 - 0.5 mol/L，以L - 天冬氨酸作为氨基供体，pH 8.5，37℃。当苯丙酮酸浓度为0.3 mol/L时，6小时后可产生48 g/L的L - 苯丙氨酸，转化率为97%。
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