[Preparation of immobilized penicillin V acylase from Fusarium oxysporum and its properties].

Extracellular penicillin V acylase from Fusarium oxysporum FP941 was partially purified by means of adsorption on, and elution from gamma-alumina and (HN4)2SO4 fractional precipitation. The enzyme was immobilized on acrylic polymer support linkaged with covalent bond. The activity of wet immobilized enzyme was 217 IU/g, The activity yield of Immobilized enzyme was 53%. Optimum pH and temperature for immobilized enzyme action wer 8.0 and 55 degrees C, respectively. The enzyme was stable below 50 degrees C and pH 4-11. The activity of the immobilized enzyme remained 90% after it was reused 25 times.