固定化青霉素G酰化酶对7-苯乙酰氨基去乙酰氧基头孢烷酸的酶促水解
Enzymatic hydrolysis of 7-phenylacetamidodesacetoxycephalosporanic acid by immobilized penicillin G acylase.
作者信息
Sudhakaran V K, Deshpande B S, Ambedkar S S, Shewale J G
出版信息
Hindustan Antibiot Bull. 1989 Aug-Nov;31(3-4):79-82.
PMID:2486271
Abstract
Enzymatic parameters such as pH, temperature and substrate concentration were studied for the hydrolysis of 7-PADCA by penicillin G acylase. Optimum pH and temperature were 8.0 and 50 degrees C, respectively. Km value of soluble and immobilized enzyme for 7-PADCA was 2.3 x 10(-5) M and 7.5 x 10(-5) M, respectively. At 7-PADCA concentration of 5% and an IME: 7-PADCA ratio of 1:2.5, the hydrolysis was complete in 110 min.
摘要
研究了青霉素G酰化酶水解7-苯乙酰氨基脱乙酰头孢菌素酸（7-PADCA）的酶促参数，如pH值、温度和底物浓度。最佳pH值和温度分别为8.0和50℃。可溶性酶和固定化酶对7-PADCA的米氏常数（Km值）分别为2.3×10⁻⁵M和7.5×10⁻⁵M。在7-PADCA浓度为5%且固定化酶与7-PADCA的比例为1:2.5时，110分钟内水解反应完成。
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