水稻黑条矮缩病毒基因组S7片段的cDNA克隆与序列分析

[cDNA cloning and sequence analysis of genome segment S7 of rice black-streaked dwarf virus].

作者信息

Zhang Hengmu, Chen Jianping, Lei Juanli, Cheng Ye, Xue Qingzhong

机构信息

College of Aagriculture and Biotechnology, Zhejiang University, Virology Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

出版信息

Wei Sheng Wu Xue Bao. 2002 Apr;42(2):200-7.

PMID:12557397

Abstract

Genome segments 7 of zhejiang and Hebei isolates of rice black-streaked dwarf virus (RBSDV) were amplified and sequenced. Segment 7 of Zhejiang isolate was consisted of 2193 nts (EMBL accession no. AJ297427) in length and that of Hebei isolate was 2190 nts (AJ297428). Both segments contained two non-overlapping open reading frames (ORFs), which encoded two polypeptides with molecular weights of 41 kD and 36 kD. These two segments shared 99% nucleotide identity, 100% and 94.4% amino acid identities of ORF1 and ORF2, shared 93.5% and 93.8% identities at nucleotide level, 98.1% (ORF1) and 96.5%/97.8% (ORF2) at amino acid level with S7 of Japanese RBSDV, and shared 85.1% and 85.3% identities at nucleotide level, 92.3% (ORF1), 85.5%/86.8% (ORF2) at amino acid level with S6 of Italian MRDV.

摘要

对水稻黑条矮缩病毒（RBSDV）浙江分离株和河北分离株的基因组片段7进行了扩增和测序。浙江分离株的片段7长度为2193个核苷酸（EMBL登录号AJ297427），河北分离株的片段7长度为2190个核苷酸（AJ297428）。两个片段均包含两个不重叠的开放阅读框（ORF），分别编码分子量为41 kD和36 kD的两种多肽。这两个片段的核苷酸同一性为99%，ORF1和ORF2的氨基酸同一性分别为100%和94.4%，与日本RBSDV的S7在核苷酸水平上的同一性为93.5%和93.8%，在氨基酸水平上的同一性为98.1%（ORF1）和96.5%/97.8%（ORF2），与意大利MRDV的S6在核苷酸水平上的同一性为85.1%和85.3%，在氨基酸水平上的同一性为92.3%（ORF1）、85.5%/86.8%（ORF2）。