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[嗜盐硫解酶基因的分子克隆及氨基酸组成分析]

[Molecular cloning and amino acid composition analysis of a halophilic thiolase gene].

作者信息

Liu Tiehan, Zhou Peijin

机构信息

Institute of Microbiology, Chinese Academy of Science, Beijing 100080, China.

出版信息

Wei Sheng Wu Xue Bao. 2002 Aug;42(4):406-10.

PMID:12557544
Abstract

5' and 3' end sequence of acaBl gene as primers, the gene of halophilic thiolase from haloarchae, Halobacterium sp. ZP-6 was cloned and its amino acid composition was calculated. Compared with non-halophilic thiolase, the halophilic thiolase contains more negative charge amino acid, less positive amino acid and less strong hydrophobic amino acid, and use preferably small side-chain amino acid. Those suggest that electrostatic screen, hydrophobic effect and surface tension all contribute to halophilic properties of thiolase.

摘要

以嗜盐古菌嗜盐栖热放线菌（Halobacterium sp. ZP-6）的嗜盐硫解酶基因acaBl的5'和3'端序列为引物，克隆了该基因并计算了其氨基酸组成。与非嗜盐硫解酶相比，嗜盐硫解酶含有更多带负电荷的氨基酸、更少的带正电荷氨基酸和更少的强疏水性氨基酸，且更倾向于使用小侧链氨基酸。这些表明静电屏蔽、疏水作用和表面张力都对硫解酶的嗜盐特性有贡献。

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