Song X Q, Fukao T, Yamaguchi S, Miyazawa S, Hashimoto T, Orii T
Department of Pediatrics, Gifu University School of Medicine, Japan.
Biochem Biophys Res Commun. 1994 May 30;201(1):478-85. doi: 10.1006/bbrc.1994.1726.
Complementary DNA for human cytosolic acetoacetyl-CoA thiolase (CT) was cloned with the use of anti-[human CT] antibody. The human CT cDNA clone (HCT10) has a 1479-bp insert and a 1191-base open reading frame encoding 397 amino acid residues. Partial polypeptide sequences from purified human CT were present in the deduced sequence. In vivo expression analysis showed that HCT10 encoded potassium-ion non-activated acetoacetyl-CoA thiolase with no 3-ketooctanoyl-CoA thiolase activity, which is characteristic for CT. The deduced amino acid sequence has a 34-57% homology with 4 other human thiolases and 4 acetoacetyl-CoA thiolases of microorganisms.
利用抗[人胞质乙酰乙酰辅酶A硫解酶（CT）]抗体克隆了人胞质乙酰乙酰辅酶A硫解酶的互补DNA。人CT cDNA克隆（HCT10）有一个1479碱基对的插入片段和一个1191碱基的开放阅读框，编码397个氨基酸残基。纯化的人CT的部分多肽序列存在于推导序列中。体内表达分析表明，HCT10编码无3-酮辛酰辅酶A硫解酶活性的钾离子非活化乙酰乙酰辅酶A硫解酶，这是CT的特征。推导的氨基酸序列与其他4种人硫解酶和4种微生物的乙酰乙酰辅酶A硫解酶有34-57%的同源性。
