Prehn L M
J Natl Cancer Inst. 1976 Apr;56(4):833-8. doi: 10.1093/jnci/56.4.833.
A highly immunogenic mouse tumor was studied with the use of microcytotoxicity test, performed at lymphoid cell:target tumor cell ratios ranging from 1:1 to 1,000:1 at various times during tumour growth and after tumor excision. Although the tumor was highly immunogenic, in vitro lymphoid cell-mediated cytotoxicity was shown only with lymphoid cells from hyperimmunized mice, i.e., mice that had "seen" the tumor more than once. Instead, the lymphoid cells harvested 2 and 3 weeks after tumor inoculation, as well as after excision of the tumor, stimulated the target cells. At 4 weeks after inoculation of the tumor, there appeared to be neither stimulation nor inhibition of the target tumor cells, though at this point the growth of this highly immunogenic tumor had been arrested in vivo.
利用微细胞毒性试验对一种高免疫原性的小鼠肿瘤进行了研究，该试验在肿瘤生长期间及肿瘤切除后的不同时间，以淋巴细胞与靶肿瘤细胞的比例从1:1到1000:1进行。尽管该肿瘤具有高免疫原性，但体外淋巴细胞介导的细胞毒性仅在经高度免疫的小鼠（即多次“接触”过该肿瘤的小鼠）的淋巴细胞中表现出来。相反，在肿瘤接种后2周和3周以及肿瘤切除后收获的淋巴细胞刺激了靶细胞。在肿瘤接种后4周，尽管此时这种高免疫原性肿瘤在体内的生长已被抑制，但似乎对靶肿瘤细胞既无刺激也无抑制作用。
