Salem A A, Douglas-Jones A G, Sweetland H M, Mansel R E
Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, Wales, UK.
Eur J Surg Oncol. 2003 Feb;29(1):25-8. doi: 10.1053/ejso.2002.1347.
Sentinel Node Biopsy (SNB) is considered an accurate method of detecting axillary lymph node status in patients with small breast cancer. Combined with an accurate and rapid histopathology tool, it could spare this group of patients unnecessary Axillary Node Clearance (ANC) with its associated hazards. Intraoperative examination of SNB for cancer cells has been investigated using both Frozen Sections (FS) and Imprint Cytology (IC) stained with different stains. This study is devoted to establish a reliable and rapid protocol for immunostaining of touch imprints from SNB.
We investigated two different EPOS (Enhanced Polymer One-Step staining--DAKO) anticytokeratin antibodies, five different tissue fixatives and different incubation periods and temperatures with both positive and negative controls.
We have developed a protocol, which produced good and consistent immunostaining of touch imprints. The initial results using this protocol are concordant with those of permanent Haematoxylin and Eosin (H&E) sections.
We propose this protocol for rapid immunostaining of touch imprints of SNB.
前哨淋巴结活检（SNB）被认为是检测小乳腺癌患者腋窝淋巴结状态的一种准确方法。结合准确、快速的组织病理学工具，它可以使这类患者免受不必要的腋窝淋巴结清扫术（ANC）及其相关风险。已使用冰冻切片（FS）和经不同染色的印片细胞学（IC）对SNB进行癌细胞的术中检查。本研究致力于建立一种可靠、快速的前哨淋巴结触摸印片免疫染色方案。
我们研究了两种不同的EPOS（增强聚合物一步染色法——丹麦戴科公司）抗细胞角蛋白抗体、五种不同的组织固定剂以及不同的孵育时间和温度，并设置了阳性和阴性对照。
我们制定了一种方案，该方案能对触摸印片产生良好且一致的免疫染色效果。使用该方案的初步结果与苏木精和伊红（H&E）永久切片的结果一致。
我们推荐此方案用于前哨淋巴结触摸印片的快速免疫染色。
