兔肾皮质中可能存在肾素原。

Possible existence of prorenin in rabbit renal cortex.

作者信息

Hiwada K, Kokubu T, Yamamura Y

出版信息

Jpn Circ J. 1976 Feb;40(2):119-22. doi: 10.1253/jcj.40.119.

DOI:10.1253/jcj.40.119

Abstract

The possible existence of prorenin (inactive form of renin) in rabbit renal cortex was studied. Renal cortex was homogenized with 0.25 M sucrose. Following centrifugation at 800 g for 10 min, the supernatant (Fraction H) was centrifuged at 20,000 g for 20 min. The pellet (Fraction G) was separated from soluble fraction (Fraction S). Intravenous injection of non-treated Fraction G caused a little blood pressure elevation in rats. However, the pressor activity of the same amount of Fraction G which was frozen and thawed 5 times previously was more than 10 times as that of non-treated one. The renin activity in preincubated fraction G with Fraction S was 2-fold the summation of the activity of renin in each fraction, when measured using indirect method. Fraction S lost not only the renin activity but also the renin activating activity when heated for 5 min at 100 degrees C. Exposure to pH 3.5 made no influence on renin activity in either Fraction G and S.

摘要

对兔肾皮质中肾素原（肾素的无活性形式）的可能存在情况进行了研究。将肾皮质用0.25M蔗糖匀浆。在800g离心10分钟后，将上清液（H组分）在20,000g离心20分钟。将沉淀（G组分）与可溶性组分（S组分）分离。静脉注射未处理的G组分在大鼠中引起轻微的血压升高。然而，相同量的先前冻融5次的G组分的升压活性是非处理组的10倍以上。当使用间接方法测量时，预孵育的G组分与S组分中的肾素活性是各组分中肾素活性总和的2倍。S组分在100℃加热5分钟后不仅失去了肾素活性，还失去了肾素激活活性。暴露于pH 3.5对G组分和S组分中的肾素活性均无影响。