[钙调节小鼠表皮角质形成细胞的生长与分化]

[Calcium-regulated growth and differentiation of the mouse epidermal keratinocytes].

作者信息

Yu Hai-Qiang, Zhou Yan, Hua Ping, Tan Wen-Song

机构信息

State Key Laboratory of Bioreactor Engineering, ECUST, Shanghai 200237, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2002 Sep;18(5):626-9.

PMID:12561213

Abstract

The effect of the calcium ion (Ca2+) on the growth and differentiation of the mouse epidermal keratinocytes cultured in serum-free medium was investigated. It was found that the optimal level of calcium ion in the medium was about 0.2 mmol/L. Under such a culture condition the colony forming efficiency, attachment percentage, percentage of the cells with cornified envelops, and percentage of the senesced cells were measured to be about 10.8%, 30.8%, 5.1%, and 26.8%, respectively. However, the Ca2+ concentrations in the medium above 0.6 mmol/L resulted in significant differentiation and senescence of the keratinocytes, which was found to be harmful for keratinocyte growth and expansion in vitro.

摘要

研究了钙离子（Ca2+）对在无血清培养基中培养的小鼠表皮角质形成细胞生长和分化的影响。发现培养基中钙离子的最佳水平约为0.2 mmol/L。在这种培养条件下，测得集落形成效率、贴壁率、有角化包膜细胞的百分比和衰老细胞的百分比分别约为10.8%、30.8%、5.1%和26.8%。然而，培养基中Ca2+浓度高于0.6 mmol/L会导致角质形成细胞显著分化和衰老，这被发现对角质形成细胞的体外生长和扩增有害。