Inokuchi S, Ueda M, Hashimoto K, Handa H, Mitomi T
Department of Critical and Emergency Medicine, Tokai University School of Medicine, Kanagawa, Japan.
Tokai J Exp Clin Med. 1991 Dec;16(5-6):223-9.
Human epidermal keratinocytes, immortalized by the introduction of SV40-adenovirus recombinants (9), were maintained in a low calcium (0.15 mM) defined medium. When differentiation was induced by a higher extracellular calcium concentration (1.5 mM), these cells altered in shape and developed stratification with formation of desmosomes, while they demonstrated limited terminal keratinization. The expression of involucrin, one of the precursor proteins of the cornified envelope, became elevated as the cell density increased, but was not affected by calcium. These results indicate that the SV40 immortalized human keratinocytes, maintained in a low calcium defined medium, partially respond to changes in extracellular calcium concentration.
通过导入SV40 - 腺病毒重组体而永生化的人表皮角质形成细胞(9)，在低钙(0.15 mM)限定培养基中培养。当通过较高的细胞外钙浓度(1.5 mM)诱导分化时，这些细胞形态改变并形成分层，伴有桥粒形成，同时显示出有限的终末角质化。随着细胞密度增加，角质包膜前体蛋白之一的兜甲蛋白表达升高，但不受钙的影响。这些结果表明，在低钙限定培养基中培养的SV40永生化人角质形成细胞对细胞外钙浓度的变化有部分反应。
