Yang Jian-Bo, Wang Xiu-Feng, Zhao Cheng-Song, Xiang Tai-He, Li Li
Key Lab of Rice Genetics and Breeding, Ministry of Agriculture, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
Yi Chuan Xue Bao. 2002 Sep;29(9):808-13.
Mitochorndrial DNA (mtDNA), nuclear DNA (nDNA), and genomic DNA (gDNA) were individually extracted from Zhenshan 97A, Xieqingzao A and A-23, which were cytoplasmic male sterile (wild abortive, dwarf abortive and BT), and from Zhenshan 97B, Xieqingzao B and BT, which were maintainers lines. Each of them was analyzed with 500 random primers by RAPD. Six fragments specific to male sterile lines were amplified. A fragment, PWH-17, associated with wild and dwarf abortive type cytoplasmic male sterility was analyzed by Southern blotting and sequencing, and also tested by SCAR. It was 1879 bp in length, which contained 6 open reading frames and 8 repeated sequences. BLAST search revealed that partial sequence in PWH-17 showed high similarity to the upstream sequence of tRNA-Asp gene in Elytrigia elongate (GenBank accession number U14335) and wheat (X13243). The similarity ratios were 97% and 100%. It was inferred that the possible position of PWH-17 in rice (wild abortive) could be located between tRNA-Asp and Cox II, which will be discussed further.
从野败型细胞质雄性不育系珍汕97A、协青早A和A - 23，以及保持系珍汕97B、协青早B和BT中分别提取线粒体DNA（mtDNA）、核DNA（nDNA）和基因组DNA（gDNA）。采用随机扩增多态性DNA（RAPD）技术，用500个随机引物对上述材料进行分析，扩增出6条雄性不育系特异片段。对其中一条与野败型和矮败型细胞质雄性不育相关的片段PWH - 17进行Southern杂交、测序及序列特异性扩增区域（SCAR）分析。该片段长1879 bp，含有6个开放阅读框和8个重复序列。BLAST检索结果表明，PWH - 17的部分序列与长穗偃麦草（GenBank登录号U14335）和小麦（X13243）中天冬氨酸tRNA基因上游序列高度相似，相似率分别为97%和100%。推测PWH - 17在水稻（野败型）中的可能位置位于天冬氨酸tRNA和细胞色素氧化酶II（Cox II）之间，对此将作进一步讨论。
