[负载α-干扰素的聚-L-乳酸层状颗粒的制备]

[Preparation of interferon-alpha-loaded poly-L-lactic acid lamellar particles].

作者信息

Li Xiongwei, Chen Xiaoli, Liu Jialin, Cao Weimin

机构信息

Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041.

出版信息

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2002 Dec;19(4):559-62.

PMID:12561346

Abstract

Lamellar particles(lamellae) were prepared by non-solvent precipitation from crystalic poly-L-lactic acid (PLLA). The PLLA lamellae exhibite a diamond or stepped irregular shape with a size range between 3-5 microns. Prepared without any surfactants and dispersing agents, the lamellar particles have clean surface, which is advantageous for the adsorption of proteins. The PLLA lamellar particles adsorbed protein cytokine interferon-alpha (IFN-alpha) with an adsorption efficiency of > or = 95%. The release of loaded IFN could continue for more than 10 days. The cell incubation experiments showed that the PLLA lamellar particles were easy to be phagocytosed by macrophages. The immunological experiments showed that the biological activity of IFN-alpha loaded on the PLLA lamellar particle was effectively retained.

摘要

通过非溶剂沉淀法从结晶聚-L-乳酸（PLLA）制备层状颗粒（薄片）。PLLA薄片呈现出菱形或阶梯状不规则形状，尺寸范围在3至5微米之间。在没有任何表面活性剂和分散剂的情况下制备，层状颗粒表面干净，这有利于蛋白质的吸附。PLLA层状颗粒吸附蛋白细胞因子α-干扰素（IFN-α）的吸附效率≥95%。负载的IFN释放可持续超过10天。细胞培养实验表明，PLLA层状颗粒易于被巨噬细胞吞噬。免疫学实验表明，负载在PLLA层状颗粒上的IFN-α的生物活性得到有效保留。