Duan Xiaohong, Guo Dagang, Xu Kewei, Wu Junzheng, Liu Bin
School of Material Engineering, Xi'an Jiaotong University, Xi'an 710049.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2002 Dec;19(4):639-41.
To explore the possibility of microencapsulation of chondrocytes in cartilage tissue engineering, immortalized manibular condylar chondrocytes (IMCCs) were microencapsuled by Alginate-polylysine-alginate (APA) method, according to air pressure shearing model. Phase contrast microscopy, trypan blue staining exclusion, cell number counting, HE staining and immunohistochemistry method were used to observe the morphology of the microencapsules, the growth character of cells, cartilage characteristics, and so on. The results showed that IMCC could survive and grow in microencapsule, and the viability rate of cells is more than 80 per cent. The diameter of microcapsule is 779 microns in average. The number of cell increased with time, and cells went into platform in about 20 days. Cells grew in clusters and cartilage specific proteoglycans and type II collagen were highly expressed. It was concluded that IMCC could form cartilage-like tissue within microencapsulation, implying that microencapsule technique might be applicable to cartilage tissue engineering.
为探索软骨组织工程中软骨细胞微囊化的可能性，根据气压剪切模型，采用海藻酸钠-聚赖氨酸-海藻酸钠（APA）法对永生化下颌髁突软骨细胞（IMCCs）进行微囊化。采用相差显微镜、台盼蓝染色排除法、细胞计数、苏木精-伊红（HE）染色及免疫组织化学方法观察微囊的形态、细胞生长特性、软骨特征等。结果显示，IMCC可在微囊内存活并生长，细胞存活率超过80%。微囊平均直径为779微米。细胞数量随时间增加，约20天时进入平台期。细胞呈簇状生长，软骨特异性蛋白聚糖和Ⅱ型胶原高表达。结论是，IMCC可在微囊内形成类软骨组织，这意味着微囊技术可能适用于软骨组织工程。
