Liang Rui-Yun, Liao Zeng-Shun, Jiang Shan-Ping, Zhang Wei, Li Jian-Guo, Zheng Dong-Hui
Department of Respiratory Medicine, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120, P. R. China.
Ai Zheng. 2003 Jan;22(1):86-90.
BACKGROUND & OBJECTIVE: The occurrence and development of tumors are controled by oncogene, antioncogene and tumor metastasis-related gene. Cyclin D1 is the expressive product of CCND1(a kind of oncogen). Vascular endothelial growth factor (VEGF) regulates the growth of neoplastic angiogenesis, which plays a role in the invasion and metastasis of tumor. The objective of this study was to detect the expression of Cyclin D1 and VEGF in non-small cell lung cancer (NSCLC), and to explore their association with the prognosis of NSCLC.
Immunohistochemistry was used to detect the expression of Cyclin D1 and VEGF in pathological tissue sections of 55 cases of NSCLC and 10 cases of non-malignant tissue from lung lesions. The relationship between the expression of Cyclin D1 and VEGF in 55 NSCLC sections and the age, sex, smoke, size of tumor, histopathological type, differentiation, stage, lymph node metastasis and survival time were analyzed statistically.
The expression rates of Cyclin D1 and VEGF in the 55 NSCLC tissue sections were 61.82% and 74.55%, respectively. In 10 cases of non-malignant tissue sections, cyclin D1 expression was not detected and VEGF expression (+/-) was only in 2 cases. The expression of Cyclin D1 and VEGF showed: (1) there was no significant difference among age, sex, histopathological type, stage, differentiation, tumor size and smoking level; (2) there were significant differences between the patients with and without lymph node metastasis; (3) there were significant differences of survival time between positive and negative expression groups. It meant Cyclin D1 and VEGF would be the poor prognostic factors.
The expression of Cyclin D1 and VEGF occurred in more than 60% cases of NSCLC, which may play a role in the biologic behavior and prognosis of NSCLC.
肿瘤的发生发展受癌基因、抑癌基因及肿瘤转移相关基因的调控。细胞周期蛋白D1（Cyclin D1）是CCND1（一种癌基因）的表达产物。血管内皮生长因子（VEGF）调节肿瘤血管生成的生长，在肿瘤的侵袭和转移中起作用。本研究旨在检测非小细胞肺癌（NSCLC）中Cyclin D1和VEGF的表达，并探讨它们与NSCLC预后的关系。
采用免疫组织化学法检测55例NSCLC病理组织切片及10例肺部病变非恶性组织中Cyclin D1和VEGF的表达。对55例NSCLC切片中Cyclin D1和VEGF的表达与年龄、性别、吸烟、肿瘤大小、组织病理学类型、分化程度、分期、淋巴结转移及生存时间的关系进行统计学分析。
55例NSCLC组织切片中Cyclin D1和VEGF的表达率分别为61.82%和74.55%。10例非恶性组织切片中未检测到细胞周期蛋白D1表达，仅2例VEGF表达为（+/-）。Cyclin D1和VEGF的表达显示：（1）年龄、性别、组织病理学类型、分期、分化程度、肿瘤大小及吸烟水平之间无显著差异；（2）有淋巴结转移和无淋巴结转移患者之间存在显著差异；（3）阳性和阴性表达组生存时间存在显著差异。这意味着Cyclin D1和VEGF可能是预后不良的因素。
超过60%的NSCLC病例中出现Cyclin D1和VEGF的表达，它们可能在NSCLC的生物学行为和预后中起作用。
