Goswami P P, Girish K S, Chaudhuri P, Tiwari V, Akare S J, Harbola P C
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India.
Indian J Exp Biol. 2002 Jan;40(1):109-10.
A gene encoding beta toxin was amplified by polymerase chain reaction from C. perfringens type C isolate and cloned in pUC 19 vector. The nucleotide sequence was identical with C. perfringens type B beta toxin gene sequence. The Southern hybridization using labelled beta toxin gene probe revealed the presence of positive signals only in beta producing C. perfingens.
通过聚合酶链反应从产气荚膜梭菌C型分离株中扩增出编码β毒素的基因，并将其克隆到pUC 19载体中。该核苷酸序列与产气荚膜梭菌B型β毒素基因序列相同。使用标记的β毒素基因探针进行的Southern杂交显示，仅在产生β毒素的产气荚膜梭菌中存在阳性信号。
