Biswas S, Kalanidhi A P, Ashok M S, Reddy G S, Srinivasan V A, Rangarajan P N
Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India.
Indian J Exp Biol. 2001 Jun;39(6):533-6.
A rabies DNA vaccine consisting of plasmid DNA expressing the rabies virus surface glycoprotein was injected (im) twice at two week interval to outbred swiss mice or Bonnet monkeys (Macaca radiata) and the levels of rabies virus neutralizing antibody (VNA) titres were examined over a one year period. In mice, the VNA titre was maintained above the minimum protective level (0.5 I.U./ml) up to 10 months after primary immunization, while in monkeys, the titre dropped below the protective level by 6 months. An anamnestic B cell response was seen in both mice and monkeys following the administration of a booster dose, 10 and 6 months after the primary immunization, respectively. These results indicate that im injection of rabies DNA vaccine induces VNA in nonhuman primates and mice unlike intradermal (id) immunization, which was shown to induce VNA only in mice but not in monkeys. This is the first report on the induction of VNA in nonhuman primates by im inoculation of rabies DNA vaccine.
将表达狂犬病病毒表面糖蛋白的质粒DNA组成的狂犬病DNA疫苗，以肌肉注射（im）方式，每隔两周给远交系瑞士小鼠或狮尾猕猴（Macaca radiata）注射两次，并在一年时间内检测狂犬病病毒中和抗体（VNA）滴度水平。在小鼠中，初次免疫后长达10个月，VNA滴度维持在最低保护水平（0.5国际单位/毫升）以上，而在猴子中，6个月时滴度降至保护水平以下。分别在初次免疫后10个月和6个月给小鼠和猴子注射加强剂量后，二者均出现了回忆性B细胞反应。这些结果表明，与皮内（id）免疫不同，肌肉注射狂犬病DNA疫苗可在非人灵长类动物和小鼠中诱导产生VNA，皮内免疫仅在小鼠中而非猴子中诱导产生VNA。这是关于通过肌肉接种狂犬病DNA疫苗在非人灵长类动物中诱导产生VNA的首次报道。
