Patial Sonika, Chaturvedi V K, Rai A, Saini M, Chandra Rajesh, Saini Y, Gupta Praveen K
Division of Veterinary Biotechnology, Indian Veterinary Research institute, Izatnagar, 243 122, India.
Vaccine. 2007 May 16;25(20):4020-8. doi: 10.1016/j.vaccine.2007.02.051. Epub 2007 Mar 15.
A bicistronic DNA vaccine against rabies and parvovirus infection of dogs was developed by subcloning rabies glycoprotein and canine parvovirus (CPV) VP2 genes into a bicistronic vector. After characterizing the expression of both the proteins in vitro, the bicistronic DNA vaccine was injected in mice and induced immune response was compared with monocistronic DNA vaccines. There was no significant difference in ELISA and virus neutralizing (VN) antibody responses against rabies and CPV in mice immunized with either bicistronic or monocistronic DNA vaccine. Further, there was significantly similar protection in mice immunized with either bicistronic or monocistronic rabies DNA vaccine on rabies virus challenge. Similarly, dogs immunized with monocistronic and bicistronic DNA vaccines developed comparable VN antibodies against rabies and CPV. This study indicated that bicistronic DNA vaccine can be used in dogs to induce virus neutralizing immune responses against both rabies and CPV.
通过将狂犬病糖蛋白和犬细小病毒（CPV）VP2基因亚克隆到双顺反子载体中，研发出了一种针对犬狂犬病和细小病毒感染的双顺反子DNA疫苗。在体外对两种蛋白质的表达进行表征后，将双顺反子DNA疫苗注射到小鼠体内，并将诱导的免疫反应与单顺反子DNA疫苗进行比较。在用双顺反子或单顺反子DNA疫苗免疫的小鼠中，针对狂犬病和CPV的ELISA和病毒中和（VN）抗体反应没有显著差异。此外，在用双顺反子或单顺反子狂犬病DNA疫苗免疫的小鼠中，在狂犬病病毒攻击后具有显著相似的保护作用。同样，用单顺反子和双顺反子DNA疫苗免疫的犬产生了针对狂犬病和CPV的相当的VN抗体。这项研究表明，双顺反子DNA疫苗可用于犬，以诱导针对狂犬病和CPV的病毒中和免疫反应。
