β-肾上腺素能受体在女性纤维肌痛患者单核细胞上的功能

Function of beta-adrenergic receptors on mononuclear cells in female patients with fibromyalgia.

作者信息

Maekawa Kenji, Twe Cheryl, Lotaif Ana, Chiappelli Francesco, Clark Glenn T

机构信息

Division of Oral Biology and Medicine, UCLA School of Dentistry, Los Angeles, California 90095, USA.

出版信息

J Rheumatol. 2003 Feb;30(2):364-8.

PMID:12563697

Abstract

OBJECTIVE

To investigate the beta-adrenergic receptors (beta AR) in patients with chronic fibromyalgia syndrome (FM). These receptors are present on circulating mononuclear cells, and activation of G-protein coupled receptors like beta AR leads to an increase in the intracellular level of cyclic aminosine monophosphate (cAMP). Therefore, cAMP levels can be used to indirectly assess the functional status of the receptor.

METHODS

Eight female patients with FM and 9 matched healthy female controls participated in this study. Blood samples were drawn from subjects' anterocubital vein in the morning. Mononuclear cells were isolated from the whole blood according to Boyüm's method. Basal and stimulated intracellular cAMP levels were determined by enzyme immunoassay. Aliquots of 10(6) cells were incubated with or without stimulation of beta-agonist isoproterenol for 5 min. Two different concentrations of isoproterenol (10(-3) M and 10(-5) M) were utilized.

RESULTS

The basal cAMP levels in patients with FM (3.02 +/- 0.44 pmol/10(6) cells) were slightly more elevated (but not statistically different; p = 0.124, Mann-Whitney U test) than that of the control group (2.26 +/- 0.39 pmol/10(6) cells). Proterenol 10(-3) M stimulation significantly increased intracellular cAMP from the basal levels in both groups (FM group, p = 0.008; control group, p = 0.011). However, isoproterenol 10(-5) M did not increase mean intracellular cAMP levels in the FM group (p = 0.74), while a significant increase was observed in the control group (p = 0.012).

CONCLUSION

These preliminary results suggest that diminished beta AR function is associated with the chronic FM state.

摘要

目的

研究慢性纤维肌痛综合征（FM）患者的β-肾上腺素能受体（βAR）。这些受体存在于循环单核细胞上，像βAR这样的G蛋白偶联受体的激活会导致细胞内环磷酸腺苷（cAMP）水平升高。因此，cAMP水平可用于间接评估受体的功能状态。

方法

8名患有FM的女性患者和9名匹配的健康女性对照参与了本研究。早晨从前臂肘前静脉采集受试者的血样。根据Boyüm法从全血中分离单核细胞。通过酶免疫测定法测定基础和刺激后的细胞内cAMP水平。将10⁶个细胞的等分试样在有或无β-激动剂异丙肾上腺素刺激的情况下孵育5分钟。使用两种不同浓度的异丙肾上腺素（10⁻³M和10⁻⁵M）。

结果

FM患者的基础cAMP水平（3.02±0.44 pmol/10⁶个细胞）比对照组（2.26±0.39 pmol/10⁶个细胞）略高（但无统计学差异；p = 0.124，Mann-Whitney U检验）。10⁻³M的去甲肾上腺素刺激使两组的细胞内cAMP均从基础水平显著升高（FM组，p = 0.008；对照组，p = 0.011）。然而，10⁻⁵M的异丙肾上腺素未使FM组的平均细胞内cAMP水平升高（p = 0.74），而对照组则有显著升高（p = 0.012）。