[猪干扰素-γ与猪囊尾蚴抗原cC1在大肠杆菌中的融合表达]
[Fusion expression of porcine IFN-gamma and Cysticercus cellulosae antigen cC1 in Escherichia coli cells].
作者信息
Guo Y, Wu D, Sun S
机构信息
Department of Medical Genetics, Second Military Medical University, Shanghai 200433.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(4):215-7.
AIM
To construct an expression vector, including a chimeric cDNA of porcine IFN-gamma and Cysticercus cellulosae antigen cC1.
METHODS
DNA fragments of porcine IFN-gamma and cC1 including linkers were generated by polymerase chain reaction (PCR). The recombinant vector pJLA-PRcC1 was constructed by inserting a chimeric cDNA of porcine IFN-gamma and Cysticercus cellulosae antigen cC1, and transformed to E. coli XL-Blue.
RESULTS
The recombinant vector was identified by restriction analysis. An inserted fragment about 1.5 kb could be found. After induction, a 52 kDa new protein band appeared in SDS-PAGE.
CONCLUSION
The fusion expression of porcine IFN-gamma and cC1 antigen in Escherichia coli cells is successful.
目的
构建一个表达载体，其包含猪干扰素-γ和猪囊尾蚴抗原cC1的嵌合cDNA。
方法
通过聚合酶链反应（PCR）产生包含接头的猪干扰素-γ和cC1的DNA片段。通过插入猪干扰素-γ和猪囊尾蚴抗原cC1的嵌合cDNA构建重组载体pJLA-PRcC1，并将其转化到大肠杆菌XL-Blue中。
结果
通过限制性分析鉴定重组载体。可发现一个约1.5 kb的插入片段。诱导后，SDS-PAGE中出现一条52 kDa的新蛋白带。
结论
猪干扰素-γ和cC1抗原在大肠杆菌细胞中的融合表达成功。
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