[利什曼原虫抗原对草原兔尾鼠婴儿利什曼原虫感染的保护作用]

[Protective effects of leishmanial antigens against Leishmania infantum infection in Lagurus lagurus].

作者信息

Chai J, Chang K P, Zuo X, Yan L, Hou Y, Zhang S, Jiang W, Zhang L

机构信息

National Hydatid Disease Centre of China, Institute for Endemic Diseases Control and Research of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 83000.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(4):237-40.

PMID:12563775

Abstract

AIM

To determine the protective effect of leishmanial surface antigens against experimental visceral leishmaniasis in Lagurus lagurus caused by Leishmania infantum.

METHODS

Recombinant surface protein (rGP63) and lipophosphoglycan (LPG) of Leishmania were used with Corinebacterium parum vaccine as ajuvant to immunize Lagurus lagurus against a challenge with virulent strain of Leishmania infantum. The efficacy of immunoprotection was observed.

RESULTS

When challenged with up to 2 x 10(7) promastigotes, the number of LD on the liver printing sections in the rGP63 + LPG + CP immunised animals was significantly decreased, the parasite reduction rate being 89.79%. LPG + CP gave a parasite reduction rate of 60.6% and rGP63/beta-galactosidase fusion protein + CP showed a parasite reduction rate of 42.45%. Purified rGP63 showed no protection. Immunization with rGP63 + LPG + CP followed by challenge inifection with 1 x 10(6), 5 x 10(6) and 1 x 10(7) promastigotes also showed significantly reduced infection rates.

CONCLUSION

A combination of rGP63 + LPG + CP antigens could provide significant immunoprotection against L. infantum challenge in L. lagurus.

摘要

目的

确定利什曼原虫表面抗原对婴儿利什曼原虫引起的拉古鼠实验性内脏利什曼病的保护作用。

方法

将利什曼原虫的重组表面蛋白（rGP63）和脂磷壁酸（LPG）与短小棒状杆菌疫苗作为佐剂一起用于免疫拉古鼠，使其免受婴儿利什曼原虫强毒株的攻击。观察免疫保护效果。

结果

当用高达2×10⁷前鞭毛体进行攻击时，rGP63 + LPG + CP免疫动物肝脏印片切片上的病变数量显著减少，寄生虫减少率为89.79%。LPG + CP的寄生虫减少率为60.6%，rGP63/β-半乳糖苷酶融合蛋白 + CP的寄生虫减少率为42.45%。纯化的rGP63未显示出保护作用。用rGP63 + LPG + CP免疫后，再用1×10⁶、5×10⁶和1×10⁷前鞭毛体进行攻击感染，也显示感染率显著降低。