[白细胞介素-6对刚地弓形虫增殖的影响]
[Effect of IL-6 on the multiplication of Toxoplasma gondii].
作者信息
Fang Y, Tan Y, Zhang Y, Li J
机构信息
Central Lab., First Teaching Hospital, Bethune University of Medical Sciences, Changchun 130021.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(2):106-8.
AIM
To observe the effect of IL-6 on the multiplication of Toxoplasma gondii.
METHODS
Peritoneal macrophages from C57BL/6 mouse were incubated with 3H-uracil-labelled T. gondii in vitro.
RESULTS
Pretreatment (but not post-treatment) of Mphi with IL-6 enhanced T. gondii multiplication in a dose-dependent manner. Pretreatment with IFN gamma resulted in active killing of parasites whereas the addition of IL-6 resulted in a partial reversal of IFN gamma-mediated toxoplasmacidal activity. Combining TNF alpha with IL-6 and IFN gamma pretreatment resulted in restoration of toxoplasmacidal activity. Addition of polyclonal anti-TNF alpha antibodies to IL-6 and IFN gamma pretreatment resulted in enhancement in the IL-6-mediated impairment of IFN gamma function.
CONCLUSION
IL-6 could enhance intraperitoneal multiplication of T. gondii and reverse IFN gamma-mediated toxoplasmacidal activity.
目的
观察白细胞介素-6（IL-6）对刚地弓形虫增殖的影响。
方法
将C57BL/6小鼠的腹腔巨噬细胞与3H-尿嘧啶标记的刚地弓形虫在体外共同孵育。
结果
用IL-6预处理巨噬细胞（而非后处理）以剂量依赖方式增强了刚地弓形虫的增殖。用γ干扰素（IFNγ）预处理导致寄生虫被有效杀伤，而添加IL-6导致IFNγ介导的杀弓形虫活性部分逆转。将肿瘤坏死因子α（TNFα）与IL-6及IFNγ预处理相结合可恢复杀弓形虫活性。向IL-6及IFNγ预处理中添加多克隆抗TNFα抗体导致IL-6介导的IFNγ功能损伤增强。
结论
IL-6可增强刚地弓形虫在腹腔内的增殖，并逆转IFNγ介导的杀弓形虫活性。
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