Xu Y, Song G, Liu S
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(3):162-4.
To investigate the dynamics of the anti-GST antibodies in rabbits immunized with recombinant 26 kDa GST of Schistosoma japonicum.
The specific antibodies were detected weekly by GST-ELISA in rabbits vaccinated with purified recombinant 26 kDa GST antigen of S. japonicum plus FCA/IFCA; rabbits immunized with 0.85% saline plus FCA/IFCA served as control group.
The specific anti-GST antibodies began to increase at week 4 post-immunization, the mean OD value in rabbits vaccinated with recombinant GST could reach 1.03 +/- 0.46 (adjuvant control group only 0.42 +/- 0.04). The experiment was completed at week 65 post-immunization when the mean OD values for specific anti-GST antibody in immunized rabbits were 0.94 +/- 0.26(0.29 +/- 0.16 in the control group).
Strong anti-GST antibody responses could be induced in rabbits vaccinated with recombinant 26 kDa GST of S. japonicum.
研究用日本血吸虫重组26 kDa谷胱甘肽S-转移酶（GST）免疫的家兔中抗GST抗体的动态变化。
每周用GST-ELISA检测用日本血吸虫纯化重组26 kDa GST抗原加弗氏完全佐剂/不完全弗氏佐剂免疫的家兔中的特异性抗体；用0.85%盐水加弗氏完全佐剂/不完全弗氏佐剂免疫的家兔作为对照组。
特异性抗GST抗体在免疫后第4周开始升高，用重组GST免疫的家兔平均OD值可达1.03±0.46（佐剂对照组仅为0.42±0.04）。免疫后第65周实验结束时，免疫家兔中特异性抗GST抗体的平均OD值为0.94±0.26（对照组为0.29±0.16）。
用日本血吸虫重组26 kDa GST免疫家兔可诱导强烈的抗GST抗体反应。
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